HPLC-UV Analysis of Phloretin in Biological Fluids and Application to Pre-Clinical Pharmacokinetic Studies
Journal Title: Journal of Chromatography and Separation Techniques - Year 2010, Vol 1, Issue 1
Abstract
A method of analysis of phloretin [3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one] in biological fluids is necessary to study the kinetics of in vitro and[i] in vivo[/i] metabolism and its concentration in natural products. A highperformance liquid chromatographic (HPLC) method was developed for the determination of phloretin in rat serum. Separation was achieved on a Chiralcel[sup]®[/sup] OD-RH column with UV detection at 288 nm. The calibration curves were linear ranging from 0.5 to 100 µg/ml. The mean extraction efficiency was >95%. Precision of the assay was <14%, and was within 10.9% at the limit of quantitation (0.5 µg/ml). Bias of the assay was lower than 14%, and was within 9.22% at the limit of quantitation. The HPLC method was successfully applied to the pharmacokinetic study of phloretin in rats.
Authors and Affiliations
Connie Remsberg, Jaime Yáñez, Karina Vega-Villa, Neal Davies, Nicole Miranda, Preston Andrews
Keywords
Related Articles
- EP ID EP124194
- DOI 10.4172/2157-7064.1000101
- Views 42
- Downloads 0
HPLC-UV Analysis of Phloretin in Biological Fluids and Application to Pre-Clinical Pharmacokinetic Studies
A method of analysis of phloretin [3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one] in biological fluids is necessary to study the kinetics of in vitro and[i] in vivo[/i] metabolism and its concentration in na...