HPTLC FINGERPRINTING ANALYSIS OF SHILAJIT: AN AYURVEDIC HERBO-MINERAL DRUG
Journal Title: Journal of Pharmaceutical and Scientific Innovation - Year 2019, Vol 8, Issue 5
Abstract
Introduction:Shilajit (Black bitumen: Asphaltumpunjabinum) is an important herbo-mineral drug in Ayurvedic system of medicine. It is a blackish brown exudate which oozes out from sedimentary mountain rocks. Due to its high cost, increased demand and difficulty in getting pure forms, adulteration of shilajitis common in raw drug industry. So quality control parameters are necessaryto scrutinize genuine shilajit.Objectives: The present work aims to develop simple quality control parameters of shilajitin terms of physico- chemical properties and HPTLC (High Performance Thin Layer Chromatography) fingerprinting.Materials & Methods:Microscopic evaluation, flame test andorganoleptic evaluation of shilajitwere done to confirm the identity. Physico-chemical parameters such as specific gravity, pH, refractive index, loss on drying, total ash, acid-insoluble ash, solubility, fluorescence analysis andqualitative analysis were carried out. Moreover, HPTLC fingerprint was developed using Camag HPTLC instrument and detectedits presence in a pharmaceutical preparation. Results:Physico-chemical parameters of shilajitwere established and HPTLC chromatogram was developed with hydro-alcoholic extract of shilajitusing the mobile phase Toluene: Ethyl acetate. This HPTLC method was also proved successful in confirming its presence in Chandraprabhagudika.Conclusion: The present study on identification, physico-chemical evaluation and HPTLC fingerprinting of shilajitprovides useful informationregarding quality control parameters and identifying parameters to substantiate and authenticate the drug and could be used for comparison of market samples to ensure its genuineness.A simple, specific and accurate HPTLC method was validated for its fingerprint analysis and detection in a pharmaceutical preparation, Chandraprabhagudika.
Authors and Affiliations
Binitha Raj RV, Mahadevan S, Rosamma MP, Rajesh KS
Keywords
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- EP ID EP657831
- DOI 10.7897/2277-4572.085153
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