HPTLC Fingerprinting of Phenolic Acids and Assessment of Antioxidant Potential of Pouzolzia bennettiana Wight a Medicinal Plant from Nilgiri Hills
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 13, Issue 3
Abstract
Aim: To screen the secondary metabolites, evaluate the antioxidant potential and identify phenolic acids of methanolic extract of leaf and stem of Pouzolzia bennettiana Wight. Study Design: The study aimed to determine the antioxidant potential using various assays and to identify the secondary metabolites using HPTLC fingerprinting. Place and Duration of Study: KMCH college of Pharmacy, between April 2015 and December 2015. Methodology: The methanolic leaf and stem extract of Pouzolzia bennettiana Wight was screened for its phytochemical components, quantitative analysis of phenols and flavonoids, antioxidant activity by DPPH, ABTS, FRAP and TAC assay. Few phenolic acids responsible for antioxidant activity was identified by HPTLC fingerprinting. Results: The important secondary metabolites such as flavonoids, tannins, terpenoids, saponins and sterols were identified in the methanolic leaf and stem extract along with primary metabolites such as carbohydrates and proteins. The total phenol and flavonoid content of leaf extract was found to be 34.65 µg and 33.36 µg/100 µg of extract respectively whereas stem extract possessed 31.74 µg and 12.58 µg/100 µg of extract respectively. The antioxidant potential of the methanol extract of leaf and stem was evaluated and the leaf extract showed significant activities in all antioxidant assays compared to the reference antioxidant whereas the stem possessed less antioxidant activity. The HPTLC fingerprinting of leaf extract revealed the presence of the following phenolic acids orcinol, ferulic acid, benzoic acid and resorcinol. The stem extract identified phloroglucinol, ferulic acid and resorcinol. Conclusion: These results suggest that Pouzolzia bennettiana Wight may act as a potential natural antioxidant offering effective protection from free radicals. The antioxidant effect may be due to the presence of phenolic acids. The phenolic acids identified possess various other applications along with antioxidant activity.
Authors and Affiliations
P. N. Preethi, G. Jeya Jothi, Aiyalu Rajasekaran, Ramasamy Arivukkarasu
Keywords
Related Articles
- EP ID EP342230
- DOI 10.9734/BJPR/2016/28661
- Views 91
- Downloads 0
Phytochemical, Nutrient Composition and Serum Lipid Lowering Effect of Xylopia aethiopica Fruit
Aims: The present study was carried out to evaluate the effects of Xylopia aethiopica on serum lipids in fed rats. Also the quantitative phytochemical and nutrient composition was investigated. Study Design: Quantit...
In vitro and in vivo Studies of Repaglinide Fast Dissolving Tablet Utilizing Solid Dispersion Techniques
Repaglinide according to the biopharmaceutics classification system is considered as class II drug, which suffers from low aqueous solubility and hence, low bioavailability. The current study concerned with improvement o...
Some Investigations on the Traditional Pharmacopoeia about Venomous Bites and Stings from Scorpions, Snakes and Spiders; in the Hina Subdivision, Far-North, Cameroon
Background: This study aimed to find out (a) which plants and animals are used in popular phyto- and zootherapy for the treatment of spiders, scorpions and snake venoms in the Hina subdivision, of the Far-North Region of...
In vitro Anticancer Activity of Selected Medicinal Plants from Oman
This paper reports in vitro anticancer activity of hexane, chloroform, ethyl acetate, butanol and water extracts obtained from twelve (12) medicinal plants including Aloe dhufarensis, Calotropis procera, Juniperus servas...
Comparative Effect of Antidepressants (Duloxetine) and NSAID (Dexibuprofen) in a New Rat Model of Chronic Pain Induced Depression Associated with Monosodium Iodo Acetate (MIA) Induced Osteoarthritis in Rats
Objective: To evaluate the role of anti-depressants (Duloxetine) and NSAID (Dexibuprofen) in a new rat model of chronic induced depression. Methods: Twenty four male wistar rats were divided into 4 groups of 6 animals e...