HPTLC Fingerprinting of Phenolic Acids and Assessment of Antioxidant Potential of Pouzolzia bennettiana Wight a Medicinal Plant from Nilgiri Hills
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 13, Issue 3
Abstract
Aim: To screen the secondary metabolites, evaluate the antioxidant potential and identify phenolic acids of methanolic extract of leaf and stem of Pouzolzia bennettiana Wight. Study Design: The study aimed to determine the antioxidant potential using various assays and to identify the secondary metabolites using HPTLC fingerprinting. Place and Duration of Study: KMCH college of Pharmacy, between April 2015 and December 2015. Methodology: The methanolic leaf and stem extract of Pouzolzia bennettiana Wight was screened for its phytochemical components, quantitative analysis of phenols and flavonoids, antioxidant activity by DPPH, ABTS, FRAP and TAC assay. Few phenolic acids responsible for antioxidant activity was identified by HPTLC fingerprinting. Results: The important secondary metabolites such as flavonoids, tannins, terpenoids, saponins and sterols were identified in the methanolic leaf and stem extract along with primary metabolites such as carbohydrates and proteins. The total phenol and flavonoid content of leaf extract was found to be 34.65 µg and 33.36 µg/100 µg of extract respectively whereas stem extract possessed 31.74 µg and 12.58 µg/100 µg of extract respectively. The antioxidant potential of the methanol extract of leaf and stem was evaluated and the leaf extract showed significant activities in all antioxidant assays compared to the reference antioxidant whereas the stem possessed less antioxidant activity. The HPTLC fingerprinting of leaf extract revealed the presence of the following phenolic acids orcinol, ferulic acid, benzoic acid and resorcinol. The stem extract identified phloroglucinol, ferulic acid and resorcinol. Conclusion: These results suggest that Pouzolzia bennettiana Wight may act as a potential natural antioxidant offering effective protection from free radicals. The antioxidant effect may be due to the presence of phenolic acids. The phenolic acids identified possess various other applications along with antioxidant activity.
Authors and Affiliations
P. N. Preethi, G. Jeya Jothi, Aiyalu Rajasekaran, Ramasamy Arivukkarasu
Keywords
Related Articles
- EP ID EP342230
- DOI 10.9734/BJPR/2016/28661
- Views 99
- Downloads 0
Leaf Extracts of Vernonia amygdalina Del. from Northern Ghana Contain Bioactive Agents that Inhibit the Growth of Some Beta-lactamase Producing Bacteria in vitro
Aims: The current study was aimed at evaluating the phytochemical profile and in vitro bacteria growth inhibitory potential of different solvent leaf extracts of V. amygdalina from northern Ghana. Study Design: Differen...
Anti-Cancerous Efficacy of Alcoholic and Aqueous Extracts from an Endemic Plant Thermopsis turcica on Liver Carcinoma
Aims: Thermopsis turcica (T. turcica) is endemic to Turkey. Its general medicinal properties are obvious, but no investigations have been made in term of its anticancerous efficacy against liver carcinoma. So, the presen...
Development and Validation of Method for Determination of Clobetasol Propionate and Salicylic Acid from Pharmaceutical Dosage Form by HPLC
Aim: A new reverse phase high performance liquid chromatography (RP-HPLC) method for the quantitative determination of Clobetasol Propionate and Salicylic Acid in ointment was developed and validated as per ICH (Internat...
Antidiabetic Activity and Toxicity Evaluation of Aqueous Extracts of Spondias mombin and Costus afer on Wistar Rats
Aims: To evaluate the antidiabetic efficacy of the aqueous extracts of Spondias mombin leaf and Costus afer stem, both individually and in combination ratios, as well as the acute and subchronic toxicities of the most ef...
Pharmacoeconomic Analysis of Direct Activity Antiviral Drugs Used for Treatment of Genotype 1 Chronic Hepatitis C
Aims: Hepatitis C is one of the most important global health care problems. The goal of this study was to perform a pharmacoeconomic analysis of therapeutic schemes used in treatment of patients with genotype 1 chronic H...