HPTLC METHOD DEVELOPMENT AND VALIDATION FOR IDENTIFICATION AND QUANTIFICATION OF LUPEOL IN THE LEAVES OF ALSTONIA SCHOLARIS
Journal Title: International Journal of Pharmaceutical Sciences and Research (IJPSR) - Year 2019, Vol 10, Issue 10
Abstract
In ancient literature plant Alstonia scholaris (Apocynaceae) has been considered medicinally important in the treatment of various ailments. Extracts of this plant have shown wide spectra of pharmacological activities like anti-cancer, hepatoprotective, anti-inflammatory, anti-diabetic, etc. These activities can be attributed to the depositories of various phytoconstituents like alkaloids, flavonoids, phenols, triterpenoids, saponins, etc. present in it. Thus, a simple and precise HPTLC method has been developed for identification and quantification of a pentacyclic triterpenoid- lupeol in the leaves of Alstonia scholaris. The developed method was validated following ICH guidelines concerning linearity, range, specificity, precision, accuracy, LOD, and LOQ. After derivatization, the optimized mobile phase Toluene: Ethyl acetate: Formic acid (7:2:1 v/v/v) exhibited good resolution of bands at 580 nm. The Rf value of lupeol was found to be 0.63 and amount quantified in ethanol and chloroform extracts of leaves was 0.31 and 0.35% w/w respectively. The calibration curve of lupeol was linear between 200-1000 ng/band with correlation coefficient 0.9990. LOD and LOQ were 55 and 166.69 ng/band respectively. Recovery was 91.88%. Thus the developed method was found to be specific, precise, and accurate for identification and quantification of lupeol in the leaves of Alstonia scholaris and can be used for routine analysis of the plant.
Authors and Affiliations
M. M. Sanaye et al.
Keywords
Related Articles
- EP ID EP656794
- DOI 10.13040/IJPSR.0975-8232.10(10).4598-04
- Views 77
- Downloads 0
DEVELOPMENT AND INVESTIGATION OF THERMO-SENSITIVE ORGANOGEL OF DICLOFENAC SODIUM FOR IN-SITU IMPLANTATION
The aim of the present study was to develop and evaluate thermo-sensitive in-situ implant forming injectable organogel systems of diclofenac sodium for prolonged drug effects. The formulations were prepared by separately...
REALISTIC DEFORMATION AND REMOVAL OF SOFT TISSUES MODELING FOR THE SIMULATION OF VIRTUAL SURGERY
Most of the simulation methods for soft tissue modeling involve tetrahedral meshes which is quite complex and takes much computation time. Instead, this work attempts to make use of delaunay triangulated mesh that consis...
MOLECULAR DOCKING STUDIES OF A FEW NOVEL PYRIMIDINE DERIVATIVES AS REVERSE TRANSCRIPTASE HIV-1 INHIBITORS
Anti-HIV drug discovery has been increasingly focusing on HIV-1- reverse transcriptase as a potential therapeutic target. Pyrimidine-based derivatives are studied for their HIV-1 reverse transcriptase inhibition activiti...
SYNTHESIS NEW MOLECULARLY IMPRINTED POLYMERS FOR THE SELECTIVE ELECTRODES OF ISOPROPAMIDE FROM PHARMACEUTICAL SAMPLES
Liquid electrodes of polymers imprinted with Isopropamide (ISP) were synthesized based on precipitation polymerization mechanism. The molecularly imprinted polymer (MIP) and non-imprinted polymers (NIP) were synthesized...
FLAVONOIDS IMPACT ON PREVENTION AND TREATMENT OF OBESITY AND RELATED METABOLIC RISK FACTORS
Metabolic syndrome, the most prevailing health concern worldwide, and their incidences are increasing at a very high rate, resulting in enormous social costs. Obesity is a measure risk factor for non-communicable disease...