HPTLC METHOD FOR THE DETERMINATION OF LUPEOL FROM ANDROGRAPHIS ECHIOIDES LEAVES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 5
Abstract
Objective: A new simple, precise, rapid and selective highâ€performance thinâ€layer chromatographic (HPTLC) method has been developed for the determination of lupeol in methanolic leaves extract of Andrographis echioides.Methods: As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of lupeol as standard on HPTLC plates for the quantification of lupeol from the Andrographis echioides leaves. The concentration of standard lupeol was 1 mg/ml.Results: The retention factor of lupeol was found to be 0.55. Linearity was obtained in the range of 5000 ngâ€10000 ng for lupeol. The developed and validated HPTLC method was employed for lupeol in methanolic leaves extract of Andrographis echioides for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99917 for lupeol. Satisfactory recoveries of 99.80 % were obtained for lupeol.Conclusion: The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of lupeol in methanolic leaves extract of Andrographis echioides.
Authors and Affiliations
S. Gurupriya, L. Cathrine, P. Pratheema
Keywords
Related Articles
- EP ID EP567506
- DOI 10.22159/ijpps.2018v10i5.24748
- Views 107
- Downloads 0
DEMOGRAPHIC, CLINICAL CHARACTERISTICS AND DRUG PRESCRIPTION PATTERN IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SOUTH INDIAN TERTIARY CARE HOSPITAL
Objective: The objective of the study was to describe demographic, clinical features and drug treatment pattern among rheumatoid arthritis (RA) patients in a south Indian tertiary care hospital.Methods: In this retrospec...
FORMULATION AND EVALUATION OF DISPERSED PERMETHRIN PRONIOSOMES IN POWDER AND MICROEMULSION-BASED HYDROGEL BASES FOR THE TREATMENT OF SCABIES
Objective: This study is aimed to encapsulation of permethrin in proniosomes and formulation and evaluation of dispersed permethrin in powder and micro emulsion-based hydrogel bases.Methods: Permethrin proniosomes were p...
PHYSICOCHEMICAL, PRELIMINARY PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY AGAINST CLINICAL PATHOGENS OF MEDICINALLY IMPORTANT ORCHID GEODORUM DENSIFLORUM (LAM) SCHLTR
Objective: To evaluate the Physicochemical, Preliminary Phytochemical analysis and Antibacterial activity of Geodorum densiflorum against clinical pathogens and to prove the orchid will target to meet the therapeutic dem...
PHARMACOKINETICS OF VANCOMYCIN IN CRITICALLY ILL PATIENTS IN THAILAND
Objective: The pharmacokinetics and pharmacodynamics of drugs in critically ill patients are difficult to predict due to complex pathophysiological changes. Vancomycin is an antibiotic commonly used to treat serious gram...
COMPARATIVE STUDY OF KANAMYCIN AND CAPREOMYCIN ON SERUM POTASSIUM LEVEL OF MULTIDRUG RESISTANCE TUBERCULOSIS PATIENTSAT A HOSPITAL IN BANDUNG, INDONESIA
Objective: Kanamycin and capreomycin are the second lines injectable ant tuberculosis used in therapy regimen of multidrug resistance tuberculosis (MDR TB) in Indonesia. Both have effects on serum potassium levels and ca...