HPTLC METHOD OF QUANTITATION OF BIOACTIVE MARKER CONSTITUENT PINITOL IN EXTRACTS OF PISONIA GRANDIS (R.BR)
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 9
Abstract
Pisonia grandis R.Br. is an evergreen medicinal plant in the four O'clock family of Nyctaginaceae. Leaves, stems and root of this plant are extensively used by tribals in India to prepare several folk medicines to treat rheumatism, arthritis and diabetes. Recently pinitol has been reported from the leaves of Pisonia grandis and thus the present study was designed to quantify this antidiabetic molecule in the various extracts of leaves of Pisonia grandis by high performance thin layer chromatography (HPTLC). The calibration curve of a stabilized HPTLC method was linear in the range of 1.5 to 7.0µg per spot and the correlation coefficient was found to be 0.9718, thus exhibiting good linearity between concentration and area. The limit of quantification (LOQ) and limit of detection (LOD) was found to be 1.5 and 1.2 µg respectively. The present study also revealed that among all the extracts, PGSX3 and PGSX4 contain more than 8 µg of pinitol per milligram of extract and thus exhibited chloroform: methanol mixture (9:1) and 100 % ethanol are found to be effective solvents to extract pinitol from Pisonia grandis. This is the first report on quantitation of pinitol by HPTLC.
Authors and Affiliations
G. Poongothai, Shubashini Sripathi
Keywords
Related Articles
- EP ID EP92827
- DOI -
- Views 113
- Downloads 0
DIRECTLY OBSERVED TREATMENT SHORT-COURSE FOR TUBERCULOSIS: A THREE-YEAR SURVEY OF THE TREATMENT OUTCOME IN A TEACHING HOSPITAL IN NORTHEASTERN NIGERIA
The major challenges of treating patients with pulmonary tuberculosis have been those of compliance and increasing incidence of multi-drug resistance. The use of directly observed treatment-short course (DOTS) has been w...
MEDICINAL PLANTS USED AS AN ANTIDIABETIC DRUG IN PHARMACEUTICAL INDUSTRY AND THEIR CONSERVATION: AN OVERVIEW
This article attempts to review the literature and the emerging policy issues on Antidiabetic plants for pharmaceutical usage. Also provides a brief review of different plants using in the traditional system...
SALT STRESS TOLERANCE AND STRESS PROTEINS IN WHEAT (Tritium Aestivum L.)
One of the unique properties of living organisms is growth. Growth is a complex phenomenon and represents the end result of metabolic pathways bringing about an overall irreversible change. Growth is the final morphologi...
ANALYSIS OF ELLAGIC ACID IN FRESH AND PROCESSED FRUIT PRODUCTS BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY
Ellagic acid, a dimeric compound of Gallic acid is found in various fruits and vegetables. They are available in the form of hydrolysable tannins called as ellagitannins. The aim of the present study is to develop and va...
A STUDY ON CYTOTOXIC AND ANTHELMINTIC ACTIVITIES OF CRUDE EXTRACTS OF LEAVES OF CLERODENDRUM VISCOSUM
This study describes the biological investigations of Clerodendrum viscosum – a plant belonging to the family Verbenaceae. The leaf of Clerodendrum viscosum was extracted with methanol and water. The crude extract of met...