HPTLC profile of aqueous extract of different chromatographic fractions of Aloe barbadensis Miller
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2012, Vol 2, Issue 0
Abstract
Objective: To establish the fingerprint profile of Aloe barbadensis Miller by using High Performance Thin Layer Chromatography (HPTLC) technique. Methods: after optimization of solvent system by Thin Layer Chromatography, different fractions of aqueous extract of Aloe was collected by column chromatography and the fingerprinting was done by High Performance Thin Layer Chromatography (HPTLC) Results: solvent system of methanol:chloroform :: 1:1 was found appropriate for fractionation by column chromatography. Total seven fractions were collected and they were analysed by HPTLC. Distinct spectra of different constituents were obtained for each fraction. Conclusions: HPTLC fingerprinting of Aloe barbadensis Miller may be useful in characterization of different phytochemicals found in this species. It may also be useful in differentiating the species from the adulterant and act as a biochemical marker for this medicinally important plant in the pharmaceutical industry.
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