hsa-miR- 574-5p Down Regulates the Expression of CBR1 in A549 Cells and Enhances their Invasive Ability
Journal Title: Journal of Pharmaceutical and Biomedical Sciences - Year 2017, Vol 7, Issue 4
Abstract
Objective To explore the impact of CBR1expression on A549 cell proliferation and invasive ability, and investigate the regulatory function of hsa-miR-574-5p on these processes. Methods A549 cells with high and low CBR1 expression were established by transfection with a CBR1-pENTER plasmid and an hsa-miR-574-5p analog, respectively, MTT assays were performed to measure cell proliferation ability, RT-PCR and western blot were used to detect the expression of CBR1, and E-cadherin, and transwell migration assays were used to detect the invasive ability of the cells. Results Cells transfected with CBR1-pENTER showed increased CBR1 and E-cadherin expression and reduced invasive ability; those transfected with hsa-miR-574-5p showed reduced CBR1 and E-cadherin expression and increased invasive ability. There was no significant difference in proliferation ability among the groups. Conclusions In A549 cells, the expression of CBR1 is irrelevant to cell proliferation ability but affects invasive ability; hsa-miR-574-5p can enhance the invasive ability of cells by down regulating CBR1 expression; hsa-miR-574-5p may not only be biological marker for diagnosis, but also may be an important prognostic index for patients with non-small cell lung cancer.
Authors and Affiliations
Aijun Liu, Zhiming Liang, Jianping Zhang
Antioxidant and Hepatoprotective Effect of Extracts of Some Commonly Consumed Local Fruits in Maiduguri
The present study assessed the in vitro antioxidant potential of Cucumis sativus, Solanum macrocarpon and Solanum aethiopicum and hepatoprotective effect of S. macrocarpon ethanolic extract in in vivo models. The antioxi...
Correlation between Osteocalcin, Non-HDL Cholesterol and Metabolic Syndrome in Postmenopausal Women
Background Osteoporosis, cardiovascular diseases (CVD), and metabolic syndrome (MS) are associated with a higher mortality rate. Bone tissues, adipocytes, and the brain interact together to control body weight and regula...
Drug-induced Delirium: A Frequent and Important Matter for Geriatricians
Use and abuse of drugs, especially if inappropriate, are among the most frequent causes of delirium in elderly people. This article describes an interesting case report on drug-induced delirium in a 74-year-old woman tak...
Acute Megakaryoblastic Leukaemia: Experience of Diagnostics at Three Clinical Centres
Background Acute megakaryoblastic leukaemia (AMkL) is a rare hematologic clonal malignancy, characterised by the accumulation of abnormal myeloid blasts with megakaryoblastic differentiation in the bone marrow and/or ext...
Placental Chorioangioma and Preterm Labour: A Case Report
We present a case of chorioangioma with associated polyhydramnios detected antenally on ultrasonography resulting in preterm labor and confirmed on histopathology postnatally.