Human Amniotic Epithelial (HAE) Cell Grafts Establish Connections with Host Hippocampal Cells and Suppress Aberrant Host Mossy Fiber Sprouting of the Dentate Supragranular Layer in a Rat Model After Trimethyltin Chloride (TMT) Lesion
Journal Title: INTERNATIONAL JOURNAL OF RECENT TRENDS IN SCIENCE AND TECHNOLOGY - Year 2014, Vol 9, Issue 2
Abstract
Introduction: The main aim of present study is to provide adequate numbers of cells to appropriate sites for useful cellular replacement to overcome the functional deficits caused by the trimethyltin chloride lesion. Methods: This study was conducted on the Wistar albino rats. Hippocampal disorder was induced by the intraperitoneal administration of trimethyltin chloride at single dose of 7.5 mg/kg body weight or two divided doses of 3.75 mg/kg body weight for two days. The human amniotic epithelial cells to be transplanted were isolated from the fetal surface of the human placenta obtained from uncomplicated elective caesarian. Using standard co-ordinates human amniotic epithelial cells were injected at four sites of hippocampal formation. For histological confirmations of the lesion and the growth of the transplant in the hippocampus, rat brains were processed and stained after various post operative periods of 7, 15, 30, 60,120 and 150 days. Results:The human amniotic epithelial cells become converted into neuron like cells and establish connections with host hippocampal cells and suppress aberrant host mossy fiber sprouting of the dentate supragranular layer. Conclusion: The present study concludes that the human amniotic epithelial cells may be used as a suitable donor tissue to alleviate various degenerative diseases in animal model before the clinical trial in humans, who are suffering from various degenerative diseases.
Authors and Affiliations
Kasaragod Venkatakrishnayya Omprakash , Kanthaiah , Rathinasamy Muthusamy , Ravisankar Periyasamy , Nagaraja Puranik , Radhakrishnan Ramesh Kumar , Matada Gurushanthaiah
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