Human chorionic gonadotropin in vitro: Effects on rat sperm motility and fertilization outcome
Journal Title: Asian Pacific Journal of Reproduction - Year 2018, Vol 7, Issue 5
Abstract
Objective: To investigate the effect of human chorionic gonadotropin (hCG) on the motility of rat sperm and the fertilization rate following in vitro fertilization. Methods: hCG concentrations of 25, 50, 75 and 100 ng/mL with incubation time of 1 h and 2 h were used in medium to study sperm motility. Then, 25 and 100 ng/mL of hCG with incubation time of 2 h were applied for in vitro fertilization. After 6 h, the number of two pronuclei was counted. Obtained data was subjected to one way ANOVA and Bonferroni-Dun hoc-post test. Results: Total motility and progressive motility of sperms were increased significantly (P<0.05) in hCG experimental group with concentration of 25 ng/L and incubation time of 2 h compared to control group, however, total motility and progressive motility of sperms were decreased significantly (P<0.05) with increasing dose of hCG. Fertilization rate was decreased significantly (P<0.05) in hCG experimental group with dose of 100 ng/mL compared to 25 ng/mL and control group. Conclusions: The effect of hCG on the rat sperm motility and the rate of their fertility is dose-dependent so that hCG with dose of 25 ng/mL leads to an increase in rat sperm motility and to some extent fertilization rate.
Authors and Affiliations
Seyed Mansour Moosavi, Hossein Barzegar Ganji, Hajar Ramezanikhah, Mitra Arianmanesh
Keywords
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- EP ID EP407740
- DOI 10.4103/2305-0500.241206
- Views 210
- Downloads 0
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