Human Papilloma Virus 16 and 18 Association in Cervical Intraepithelial Lesions and Cervical Cancers by In Situ Hybridization
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2017, Vol 6, Issue 3
Abstract
Objective: To correlate the association of high risk Human Papilloma Virus (HPV) 16, 18 in cervical intraepithelial lesions and cervical cancers by in-situ hybridization (ISH) technique. Study Group: Cervical biopsy and hysterectomy specimen of 78 young and adult women, attending Hi-Tech Medical College and Hospital, Bhubaneswar, who were clinically or cytologically suspected of cervical intraepithelial lesion or cervical cancer were taken as source of target viral DNA. Material: Formalin 10% as fxative H & E stain as routine staining agent In-situ hybridization kit for HPV 16 and 18 DNA. Method: After following standard protocol for surgical grossing, HPV 16, 18 In-situ hybridization kit was used on paraffn embedded tissue sections. Results: The percentage of positive cases was highest in cervical cancer patients followed by cervical intraepithelial lesions, high grade, and low grade. Conclusion: This study has been carried out for the frst in our state and our results show high degree of positivity of HPV 16/18 in females with cervical intraepithelial lesions and cervical cancers attending our tertiary care hospital.
Authors and Affiliations
Mohanty Manisa| Department of Pathology, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India, Corresponding e-mail: mohantymanisa@gmail.com, Mohanty Ramesh Chandra| Department of Pathology, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India, Mohanty Raghumani| Department of Pathology, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India, Swain Rajeeb| Institute of Life Sciences, Bhubaneswar, Odisha, India
A RARE CASE OF MALIGNANT PERIPHERAL NERVE SHEATH TUMOUR
Malignant Peripheral Nerve Sheath Tumours are tumours of ectomesenchymal origin often originating from major nerves or their nerve sheaths, they are commonly found in patients with neurofibromatosis-1 though sporadic c...
PROSTHETIC MANAGEMENT OF PATIENT WITH PAPILLON-LEFEVRE SYNDROME: A CLINICAL REPORT
Papillon Lefevre syndrome is a rare autosomal recessive genetic disorder, which is characterized by palmarplantar hyperkeratosis, with rapidly progressive periodontitis and premature loss of both deciduous and permanent...
Efficiency of mobile dental unit in public health programs
Almost all dental Colleges run a mobile dental operation for people living in far inaccessible areas who are not able to avail dental care. Mobile dental clinics provide a mode of reaching the unreached by delivering de...
Knowledge and performance of dental students with regard to infection control guidelines in Dental School of Mazandaran University of Medical Sciences in 2015
Dentists are at risk of infectious diseases and dental offices can serve as a source of infection transmission if the infection control guidelines are not properly implemented. Adherence to infection control principles...
The study of Job Stress and Tension Management among Oncology Nurses of Ahvaz Hospitals in 2015
Nursing care for cancer patients is stressful for nurses and to maintain the quality of care they should be able to manage these tensions. Therefore, the aim of current study was to determine the level of job stress and...