Human Papilloma Virus 16 and 18 Association in Cervical Intraepithelial Lesions and Cervical Cancers by In Situ Hybridization
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2017, Vol 6, Issue 3
Abstract
Objective: To correlate the association of high risk Human Papilloma Virus (HPV) 16, 18 in cervical intraepithelial lesions and cervical cancers by in-situ hybridization (ISH) technique. Study Group: Cervical biopsy and hysterectomy specimen of 78 young and adult women, attending Hi-Tech Medical College and Hospital, Bhubaneswar, who were clinically or cytologically suspected of cervical intraepithelial lesion or cervical cancer were taken as source of target viral DNA. Material: Formalin 10% as fxative H & E stain as routine staining agent In-situ hybridization kit for HPV 16 and 18 DNA. Method: After following standard protocol for surgical grossing, HPV 16, 18 In-situ hybridization kit was used on paraffn embedded tissue sections. Results: The percentage of positive cases was highest in cervical cancer patients followed by cervical intraepithelial lesions, high grade, and low grade. Conclusion: This study has been carried out for the frst in our state and our results show high degree of positivity of HPV 16/18 in females with cervical intraepithelial lesions and cervical cancers attending our tertiary care hospital.
Authors and Affiliations
Mohanty Manisa| Department of Pathology, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India, Corresponding e-mail: mohantymanisa@gmail.com, Mohanty Ramesh Chandra| Department of Pathology, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India, Mohanty Raghumani| Department of Pathology, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India, Swain Rajeeb| Institute of Life Sciences, Bhubaneswar, Odisha, India
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