Hydrogen production and metabolic response of Spirulina sp , Calothrix parteniana and Oscillatoria sp to sulphur and /or nitrogen deficiency
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2015, Vol 6, Issue 3
Abstract
Spirulina platensis SAG obtained from (Sammlung von Algen culturing Gottingen), Calothrix parteniana and Oscillatoria sp. (isolated from Egyptian soil sample) were cultivated in BG11 medium either in the presence of nitrogen and sulphur (control culture condition) or without addition of nitrogen (N0) or sulphur (S0) and combined nitrogen and sulphur (N0S0) source to detect the effect of these element limitation on growth and hydrogen production of the tested algal isolates . The growth expressed as dry weight and chlorophyll content was generally inhibited in treated culture sample (N0 and /or S0) of all sp under study. Photosynthetic O2 evolution expressed as µ mol O2†‘/mg Chll.a/h. of Spirulina sp and Calothrix parteniana,Oscillatoria sp were severely dropped under nitrogen deficiency compared with control and sulphur limited growth condition. Respiratory oxygen uptake was however, increased with the above deficiency effect in all investigated spp. Assay of Hydrogen production using hydrogen electrode (expressed as nmol H2†‘/mg.chla/h) revealed that nitrogen or sulphur deficiency growth condition stimulated hydrogen production in all isolate under testing. Combination of both element deficiencies has an inhibition effect on hydrogen yield in Calothrix parteniana and Oscillatoria sp. Amazingly our results indicate that Spirulina sp was the only culture able to produce hydrogen in combined nitrogen and sulphur deficiency growth condition, Spirulina produce hydrogen in dark condition only, while the presence of light was very important for hydrogen production in other investigated cyanobacterial cultures. The above effect of nitrogen and sulphur deficiency demonstrate that both elements play a crucial role in growth and metabolism especially hydrogen production.
Authors and Affiliations
Awatief F. Hifney, Refat Abdel-Basset
Keywords
Related Articles
- EP ID EP654488
- DOI 10.24297/jab.v6i3.5341
- Views 154
- Downloads 0
Contribution to the knowledge of the Rubiaceae of the Lesser Antilles: Martiniques case.
The Rubiaceae represent one of the worlds five largest families of flowering plants. This family is diversified in terms of number of species, genera, physiognomic types or chemical spectra. Within their flora the Lesser...
In Vitro Activities of Essential Oils and fractions of Two Cameroonian Plants against Tomato Wilt Fusarium oxysporium lycopersici.
In this study we surveyed the effectiveness, Minimum inhibitory concentration (MIC) and Minimum fungicide concentration (MFC) of essential oils from two medicinal plant Callistemon citrinus and Tephrosia. vogelli on myce...
Validation of a growth estimation method: Application to the flathead mullet, Mugil cephalus in the Northern coast of Senegal
Growth parameters estimation (Linf and K) of Mugil cephalus in the Northern coast of Senegal was made with the ELEFAN in R program, based on biological sampling carried out on three landing sites (Kayar, Fass Boye,...
Laminaria Japonica polysaccharide promotes the expression of calcitonin receptor-like receptor in type 2 diabetes mellitus model mice
Amylin, or islet amyloid polypeptide (IAPP), is a 37-residue peptide hormone[1-2]. It is cosecreted with insulin from the pancreatic β cells in the ratio of approximatrly 100:1[3-4]. Amylin receptor (AR) is compos...
Mating system and gene flow in the Lutheran communities from historical Polish territories (the Parish of Trzebosz, the second half of the 19th and early 20th century)
The aim of this paper was to analyse the mating system and the degree of genetic isolation of the Lutheran Parish of Trzebosz situated on the borderland between Greater Poland and Silesia. Use was made of 343 records fro...