Hypertriglyceridemia but not hypercholesterolemia induces endothelial dysfunction in the rat.
Journal Title: Pharmacological Reports - Year 2005, Vol 57, Issue
Abstract
In humans, hypercholesterolemia and hypertriglyceridemia induce endothelial dysfunction and therefore lead to atherosclerosis. In contrast, rats are resistant to atherosclerosis. Here we analyze whether rats respond to hypercholesterolemia and hypertriglyceridemia by developing of endothelial dysfunction. To induce hypercholesterolemia Wistar-Kyoto (WKY) and spontaneous hypertensive(SHR) rats were fed for 12 weeks with AIN93 diet supplemented with cholesterol (1%) and butter (20%). To induce hypertriglyceridemia Wistar were fed for 8 weeks with AIN93 diet supplemented with 60% fructose. In all experimental groups nitric oxide (NO)-dependent and prostacyclin (PGI(2))-dependent function was assessed in the isolated aorta. Additionally in hypertriglyceridemic rats endothelial function in the isolated mesenteric resistance artery was analyzed. NO-dependent vasodilation induced by acetylcholine or histamine in aorta of SHR and WKY rats was modestly impaired. Hypercholesterolemic diet fed to WKY and SHR rats induced a rise in total cholesterol and low-density lipoproteins (LDL) cholesterol by 2.5 and 4.5 fold, respectively, but did not further impair NO-dependent vasodilation. Although basal production of PGI(2) in aortic rings from SHR rats was five fold higher than in aortic rings from WKY rats, the hypercholesterolemic diet did not further affect aortic PGI(2) production in either rat strain. Endothelium-independent vasodilation induced by SNAP remained also unchanged. On the other hand, the hypertriglyceridemic diet given to Wistar rats led to a selective 1.5-2 fold elevation of triglycerides that was associated with the impairment of NO-dependent relaxation in aorta as well as in the mesenteric resistance artery. Interestingly, the basal production PGI(2) by aortic rings was not modified by hypertriglyceridemic diet. Again endothelium-independent relaxation induced by S-nitroso-N-acetyl-penicilamine (SNAP) was not affected. In summary, although in humans both hypercholesterolemia and hypertriglyceridemia are associated with endothelial dysfunction, in rats hypertriglyceridemia only led to the impairment of NO-dependent vasodilation. Hypercholesterolemia did not modify endothelial function even in hypertensive rats that display pre-existing alterations invasodilator function.
Authors and Affiliations
Magdalena Bartuś, Barbara Lorkowska, Renata Kostogrys, Paweł Pisulewski, Stefan Chłopicki
Keywords
Related Articles
- EP ID EP133847
- DOI -
- Views 125
- Downloads 0
Thiopurine S-methyltransferase phenotype-genotype correlation in hemodialyzed patients.
Thiopurine S-methyltransferase (TPMT) is a cytosolic enzyme, catalyzing S-methylation of thiopurine drugs. TPMT exhibits autosomal codominant polymorphism. Patients carrying a variant genotype have low TPMT activity, and...
Distinct hydrogen peroxide-induced constriction in multiple mouse arteries: potential influence of vascular polarization.
It is a matter of controversy whether the reactive oxygen species hydrogen peroxide (H(2)O(2)) contributes to tone in the vasculature as a vasodilator or vasoconstricting factor. To address this, we hypothesized that H(2...
Different effects of perindopril and enalapril on monocyte cytokine release in coronary artery disease patients with normal blood pressure.
Favorable effects of angiotensin-converting enzyme (ACE) inhibitor treatment on the incidence of cardiovascular and cerebrovascular mortality and morbidity are not limited to patients with elevated blood pressure. As sug...
Opposite effects of beta amyloid on endothelial cell survival: role of fibroblast growth factor-2 (FGF-2).
Amyloid beta-peptides (Abeta) aggregate during Alzheimer's disease contributing to the development of the pathology. At micromolar concentration Abeta is toxic and accumulates in neurons and in the vasculature. However,...
Huntington's disease: pathogenesis to animal models.
Huntington's disease (HD) is an inherited genetic disorder, characterized by cognitive dysfunction and abnormal body movements called chorea. George Huntington, an Ohio physician, described the disease precisely in 1872....