Identifiation and determination of the prevalence of Toxoplasma gondii in patients with chronic renal failure by ELISA and PCR
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2016, Vol 6, Issue 5
Abstract
Objective: To detect Toxoplasma gondii (T. gondii) among end-stage renal disease (ESRD) patients. Methods: This case-control study was conducted on 180 blood samples. In compliance with all ethical principles, 90 blood samples were taken from hemodialysis patients with ESRD and 90 samples from healthy volunteers. T. gondii screening was done using ELISA to search for immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies and by PCR for amplification of the T. gondii genome using specific primers. Results: The results were analyzed using SPSS software. Out of 90 patients on hemodialysis, 54 (60.0%) were positive for anti-toxoplasma IgG antibody, 3 (3.3%) for anti-toxoplasma IgM antibody and 5 patients (6%) were positive by PCR. From 90 healthy volunteers, 34 (37.8%) were positive for anti-toxoplasma IgG antibody. All the healthy volunteers were negative for anti-toxoplasma IgM antibody and in PCR. Compared with the gold standard method of ELISA, PCR had 100% sensitivity and 98.9% specificity in detection of T. gondii. Conclusions: PCR alongside serologic methods can be valuable for T. gondii screening. Given the high prevalence of T. gondii among hemodialysis patients with ESRD, T. gondii screening together with sanitary control of biological agents was recommended in dialysis units.
Molecular detection of Anaplasma species in questing ticks (ixodids) in Ethiopia
Objective: To identify Anaplasma spp. in questing ticks with emphasis on Anaplasma phagocytophilum (A. phagocytophilum) and Anaplasma ovis (A. ovis) in Ethiopia. Methods: DNA extracted from 237 questing ticks [Rhipicepha...
Retrospective analysis of dengue specific IgM reactive serum samples
Objective: To conduct a retrospective analysis of dengue cases in Kolkata, on the basis of presence of anti-dengue IgM in their sera and presence or absence of anti-dengue IgG and dengue specific Non structural 1 (NS1) a...
Species composition, sex ratio, geographical distribution, seasonal and monthly activity of scorpions and epidemiological features of scorpionism in Zarrin-dasht County, Fars Province, Southern Iran
Objective: To determine the fauna, density, geographical distribution, sex ratio of scorpions and epidemiology of scorpionism in Zarrin-dasht County, south of Iran. Methods: This descriptive and practical research was do...
Efficiency of Tribulus terrestris L. as an antibiotic growth promoter substitute on performance and immune responses in broiler chicks
Objective: To evaluate the effect of Tribulus terrestris L. (puncture vine) as an antibiotic growth promoter substitute on growth performance, carcass traits, and immune responses in broiler chickens. Methods: A total of...
Entamoeba spp. diagnosis in patients with inflmmatory diarrhea by staining, copro-antigen ELISA and multiplex PCR methods
Objective: To evaluate Entamoeba spp. diagnosis in patients with inflammatory diarrhea by staining, copro-antigen ELISA and multiplex PCR methods. Methods: In this descriptive cross-sectional survey, 200 stool samples we...