IDENTIFICATION ACTIVE COMPOUNDS OF BACTERIA STREPTOMYCES USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
Journal Title: World Journal of Pharmaceutical and life sciences - Year 2017, Vol 3, Issue 6
Abstract
Aim of the study assess optimum Growth of Streptomyces and identification active compounds of bacteria Streptomyces using HPLC. The biochemical and physiological testes of Streptomyces sp. Show that the Streptomyces produced extra cellular enzymes like amylase, urease, catalase, lipase, protease, pectinase, cellulase and phosphatase, no HCN production and negative for indole production. Estimating the stationary phase for isolated Streptomyces were growing on malt extract yeast extract broth ( ISP2), and getting the optical density (OD) at 600nm during (1-7) days and optimal growth of them ranged (6.5 to 8), while were salt concentrations ranged from (1 to 7% NaCl). The best growth happened at 30 °C by measuring the absorbance of the growth and getting the optical density (OD) at 600nm. We watched the growth state activity of isolated strain which started after 72 hours of incubation at 30°C, when the OD was about (0.572 ). The HPLC analysis of extracellular crude extract was showed found five different antibiotic (Tetracycline, Streptomycin, Neomycin, Vancomycin and Kanamycin).
Authors and Affiliations
Dr. Mohsen Hashim Risan
Keywords
Related Articles
- EP ID EP649320
- DOI -
- Views 140
- Downloads 0
REGULATION OF BREASTFEEDING PATTERNS BY POSTNATAL MOTHERS
Maternal attitudes toward breastfeeding have impact on when they commence and how long they practice breastfeeding. This study examined how postnatal mothers regulate their breastfeeding patterns. Convenient sampling met...
UTILITY OF ASHTA AHARA VIDHI VISHESHAYATANA IN PRESENT ERA
Rapid changes in diet and lifestyle led to a major rising shift towards diabetes, obesity, arthritis, mental illness, cardiac diseases, cancer etc. Ayurvedic principles certainly play an important role in their preventio...
IXEKIZUMAB: A MONOCLONAL ANTIBODY WELL WORTH FOR PSORIASIS
Psoriasis is a very long lasting disease affecting millions of people worldwide. In the patients affected by psoriasis, the skin cells multiply faster than normal and raised and red plaques appeared over the skin. Psoria...
EVALUATION OF EFFECTIVENESS OF TAGETES ERECTA EXTRACT IN CONTROLLING CITRUS NEMATODE TYLENCHULUS SEMIPENETRANS IN WASIT PROVINCE
The study has been conducted in the laboratory of college of science – university of wasit in cooperation with the ministry of science and technology- directorate of agriculture research to evaluate the effect plant extr...
IN SILICO STUDIES ON DENGUE AND ZIKA VIRAL PROTEINS WITH SELECTED EMBLICA OFFICINALIS GAERTN CONSTITUENTS
Medicinal plants are the precious gift of nature which plays vital role in health care sector for developing nation and potent source of therapeutic molecules to heal various diseases in the world. Emblica officinalis Ga...