Identification and characterization of Pseudomonas syringae pv. syringae strains from various plants and geographical regions
Journal Title: Journal of Plant Protection Research - Year 2018, Vol 58, Issue 4
Abstract
Pseudomonas syringae pv. syringae (Pss) constitutes a diverse group of bacterial strains that cause canker of stone fruits, blight of cereals and red streak of sugarcane. The purpose of this study was to determine how diverse Iranian strains of Pss are when they come from different hosts. We compared a total of 32 Pss strains isolated from stone fruits, barley, wheat and sugarcane from different geographical regions of Iran based on their phenotypic and molecular properties. Strains showed some variation regarding carbon and nitrogen utilization. Pss strains were similar in their protein banding patterns. Additional bands were found in sugarcane strains. Most strains showed one indigenous plasmid DNA and a few had two and some none. The genes of syrB and syrD encoding syringomycin synthesis and secretion, respectively, were amplified using specific primers in polymerase chain reaction. Syringomycin, producing strains amplified two DNA fragments of 752 and 446 bp representing syrB and syrD genes, respectively. Primer specificity was shown for Pss using various genera. Based on the results of this study, it is suggested that Pss strains from different hosts and geographical regions show diversity in phenotypic and molecular characters. It is thought that phenotypic variation is due to adaptation to specific hosts and niches for survival and pathogenicity.
Authors and Affiliations
Maryam Khezri, Mojtaba Mohammadi
Keywords
Related Articles
- EP ID EP546113
- DOI 10.24425/jppr.2018.124647
- Views 76
- Downloads 0
Pest risk analysis on Xylella fastidiosa in Morocco
Morocco is basically an agricultural country; almost 40% of the workforce is employed in this sector. Xylella fastidiosa is a xylem-inhabiting pathogen which can infect more than 300 plant species, although most host spe...
Glyphosate in agronomic performance and seed quality of soybean with cp4-EPSPs and cry1Ac genes
The “second generation” of glyphosate-tolerant soybean (GT2 soybean) was developed through a different technique of insertion of the glyphosate-insensitive EPSPs gene, in comparison with “first generation” of glyphosate-...
Geographic distribution of Fusarium culmorum chemotypes associated with wheat crown rot in Iraq
Fusarium crown rot (FCR) is an important disease of wheat and other grains that has had a significant impact on cereal crop production worldwide. Fusarium species associated with FCR can also produce powerful trichothece...
Production, characterization and iron binding affinity of hydroxamate siderophores from rhizosphere associated fluorescent Pseudomonas
Fluorescent Pseudomonas (FP) is a major group of plant growth promoting rhizobacteria and a well-known synthesizer of siderophores, which imparts a selective advantage on rhizosphere competence and their biocontrol trait...
Antifungal activity of Bunium persicum essential oil and its constituents on growth and pathogenesis of Colletotrichum lindemuthianum
Anthracnose disease caused by Colletotrichum lindemuthianum (Sacc. and Magnus) Lams-Scrib is one of the most devastating seed-borne diseases of common bean (Phaseolus vulgaris L.). In the present study, we evaluated the...