Identification and cloning of PIP1 gene in Carrizo citrange (Citrus sinensis × Citrus trifoliate)
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 7, Issue 4
Abstract
The genus Citrus (Rutaceae) is economically very important and is grown in tropical and subtropical areas of the world. One of the most important factors in raisingthe quantity and quality of citrus production is sufficient water. Aquaporinsare integral membrane pore proteins and conduct water molecules in and out of the cell. Plant aquaporins divided into four subgroups including plasma membrane intrinsic proteins (PIPs), tonoplast membraneintrinsic proteins (TIPs), nodulin-26–like intrinsic proteins (NIPs) and small basic intrinsicproteins (SIPs). The present study was carried out for identification of PIP1 gene in Carrizo citrange (Citrus sinensis×Citrus trifoliate). From results, a fragment with 867- bais-pair (bp) lengthwith high similarity to PIP1gene in plants was identified as a probable member of the PIP gene family. The cloned cDNA sequence has been submitted to Gen Bank under the accession number KJ546461.1.This gene is encoding a deduced protein containing 288 amino acids. The three-dimensional structural model of the protein was also constructed by SWISS-MODEL server, indicating that the gene structure of PIPs has been highly conserved.
Authors and Affiliations
E. Gholivandan, M. R. Dadpoor, A. Movafeghi, F. Zaree Nahandi, D. Zare Haghi, M. Kosari-Nasab
Keywords
Related Articles
- EP ID EP38637
- DOI -
- Views 151
- Downloads 0
The application of virtual water in order to escape the drought crisis in line with water resources management
Being located in arid and semi-arid area and increasing water consumption, Iran is going to face the threat of water crisis in the coming years. So, in order to deal with this problem care must be taken in treating diff...
Fish biodiversity and conservation status of the Dhepa river sanctuary in protection and restoration of threatened fishes of the northwest Bangladesh
A fish sanctuary has established at the Dhepa river basin in Dinajpur district of Bangladesh to protect the threatened fishes. The present fish biodiversity status and the impacts of the sanctuary on the protection and r...
Modification of chitosan radiation-induced graft copolymerization of acrylonitrile onto chitosan
The monomer, acrylonitrile, was graft copolymerized onto Chitosan using γ-rays as initiator. The reactions were carried out in a homogenous aqueous medium. After removal of the homopolymer, the graft copolymer was char...
Breeding Biology of Barn Swallow (Hirundo rustica) at Tehsil Balakot, Pakistan
This study was conducted at Tehsil Balakot in District Mansehra, during the breeding season February to June 2014. Details about nests and eggs characteristics are provided. All nests were attached to vertical walls and...
The development of new cities on environmental impact assessment
Environment effects evaluation can be used by planners and urban development managers as a tool for recognition survey and comparison of different urban development effects. the suitable effect evaluation method for th...