Identification and complete genome sequencing of human adenovirus type 55 isolated from a patient with acute hemorrhagic conjunctivitis
Journal Title: Shanghai Journal of Preventive Medicine - Year 2023, Vol 35, Issue 4
Abstract
ObjectiveTo determine the genomic characteristics of a subgenus B human adenovirus strain isolated in Shanghai in 2021.MethodsAn adenovirus type 55 strain was isolated and identified from a patient with acute hemorrhagic conjunctivitis ï¼AHCï¼. Complete genome of the strain was obtained using the next-generation sequencing ï¼NGSï¼. Phylogenetic trees were reconstructed based on the sequences of Hexonï¼ Fiberï¼ Penton and complete genome to genomically characterize this strain.ResultsPhylogenetic analysis based on the complete genome classified this strain ï¼MH2021001ï¼ into subgenus Bï¼ subspecies B2 of HAdV-55. Hexon gene of MH2021001 had close phylogenetic relationship with HAdV-11ï¼ while Fiber and Penton genes had close relationship with HAdV-14. The MH2021001 showed high nucleotide identity with currently prevalent HAdVâ55 strains ï¼>99.90%ï¼. The complete genome had 99.96% nucleotide identity to the 73-GD_CHN_2016 strain isolated in Guangdong. In additionï¼ the amino acid sequence of MH2021001 had several substitutions in regions coding for E1Bï¼ L4ï¼ E3 and L5.ConclusionThis strain has been classified to HAdV-B55. No recombination event is identified in the complete genome. Due to multiple amino acid substitutionsï¼ the biological characteristics of the strain need to be further identified.
Authors and Affiliations
LIU Jiajing,CUI Xiaoqing,ZHANG Wanju,FANG Fanghao,PENG Yajun,CHEN Min,ZHANG Jingyi,TENG Zheng,
Keywords
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