Identification and selection of somatic hybrids obtained by protoplast fusion
Journal Title: Biotecnología Vegetal - Year 2009, Vol 9, Issue 2
Abstract
Efficient selection of hybrid plants produced by protoplast fusion is very important. Several advances have been made on the subject in recent decades. The methods used for the identification of somatic hybrids include selection by morphological characteristics (growth, leaf, stem, flower or fruit type, flower color or type of inflorescence), micromanipulation (or manual selection), flow cytometry, complementation, chromosome counting, isozyme detection and the use of molecular markers, such as random amplification of polymorphic DNA (RAPD), genomic in situ hybridization (GISH), simple sequence repeats or microsatellites (SSR), the inter-simple sequence repeats (ISSR), restriction fragment length polymorphisms (RFLP) and amplified fragment length polymorphisms (AFLP). Isozyme analysis was a very common selection method for many years. Although, molecular techniques based on DNA have become reliable methods to detect somatic hybrids. Flow cytometry offers a more rapid and sensitive method for the selection of hybrid cells. However, the equipment needed for its implementation is very expensive. Use of more than one method for early detection of somatic hybrids increases the selection efficiency. This review describes the methods used for detection of somatic hybrids and presents a list of examples on their use from 1974 on.
Authors and Affiliations
Wayner Montero-Carmona, Víctor M. Jiménez
Keywords
Related Articles
- EP ID EP683855
- DOI -
- Views 139
- Downloads 0
Bacterial contamination management on in vitro propagation of axillary buds of Colocasia esculenta cv. ‘INIVIT MC-2012’
La propagación in vitro de Colocasia esculenta se limita por la presencia de contaminantes microbianos, especialmente bacterias. El objetivo de este trabajo fue demostrar que la inclusión de acciones para el manejo de la...
Use of temporary immersion systems for the in vitro multiplication of shoots of Saccharum spp. var. IBP 89-112
With the objective to increase the efficiency in the in vitro multiplication of the sugarcane (Saccharum spp. var. IBP 89-112), temporary immersion systems with a capacity of 3.7 liters were used and the effect of the in...
In vitro organogenesis in Sophora toromiro from nodal segments
Sophora toromiro is an arboreal endemic to Chile and extinct in its natural state, this specie has problem for its conservation due to its difficult propagation. The present study aimed to determine the organogenic respo...
Effect of the intercellular extracts of banana leaves inoculated with Mycosphaerella fijiensis Morelet, on the electronic transport in chloroplasts of the cv Grande naine (AAA)
Some foliar pathogens colonize intercellular spaces of damage tissues during infection process, mediated by toxins production and diffusion to kill adjacent healthy cells. Due to the absence of reliable bioassays, the ph...
Influence of the carbon source and the gelling agent on the regeneration of Indica rice variety IACuba-28 from callus
Several variations on plant regeneration medium from callus in IACuba-28 rice cultivar were evaluated using the basal MS medium with the same grouth regulator concentrations. As carbon source 30g.l -1 of maltose or sucr...