Identification and Study of cis regulatory elements and phylogenetic relationship of TaSRG and other salt response genes
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2014, Vol 4, Issue 7
Abstract
Salt stress is the major limiting factor in agriculture that affects on all developmental stages including germination, seedling and vegetative stages. Under salt stress, induce the expression of many plant genes. These genes encode the defending proteins, and regulatory proteins that regulate the stress signal transduction. The TFs interact with cis-elements in the promoter regions of several stress-related genes and thus up-regulate the expression of many downstream genes resulting in imparting abiotic stress tolerance. The identification of cisregulatory DNA elements responsive to stress is important for studying gene regulation and for biotechnological applications. In this study, we identified cis-regulatory elements and also investigated the phylogenetic relationship of salt response genes of wheat, rice, barley, maize and Arabidopsis. The result of phylogenetic tree showed that TaSRG, LTP and IPK2 genes have located in one branch. The analysis of 5´UTR of salt response genes showed four elements have the most frequencies that including, TATA Box (24.55%), Unnamed-4 (24.55%), CAAT Box (17.43%) and SP1(5.33%), respectively. The TaSRG gene also is a cis elementsrich gene in which TATA Box (5'-TATAAA-3') and CAAT (5'-CAAT-3') Box have most frequencies. The most important identified cis regulatory elements in response to stress are ABRE, G-box, MBS, TGA-element. This results indicated that gene expression control in different checkpoints of cell cycle may be dependent to both the frequency of enhancers and stress responsive cis regulatory elements under abnormal conditions.
Authors and Affiliations
Sirvan Saeediazar, Hamid Najafi Zarrini, Gholamali Ranjbar, Parviz Heidari
Keywords
Related Articles
- EP ID EP37760
- DOI -
- Views 133
- Downloads 0
Soybean yield under water deficit conditions
Drought stress is the main environmental factor that limiting crops growth and productivity in Iran. Therefore, in order to evaluate effects of water deficit on yield and yield components of soybean, an experiment was p...
Serum Copper and Zinc level as biomarker for dust exposed lung diseases among coal miners
To elucidate the effect of dust exposure, smoking and alcoholic habit on the serum Copper and Zinc activity as prognosis tool for dust linked lung diseases among coal workers. In the present study, serum Copper and Zinc...
The effectiveness of combination Mahogany (Swietenia mahogany) seed and Sour Sup (Annona muricata) leaf pesticide to the time of stop feeding and LC50 Mortality on Armyworm (Spodoptera litura F.)
Spodoptera litura Fabr. is one of polyphagous insect that attacks lot of crop species. Integrated Pest Management (IPM) is a effort to suppress insect pest population. Utilization of plant materials as ingredients insec...
Removal of Pb(II), Ni(II) and Cu(II) metal ions from industrial wastewater by nanocomposite hydrogel base starch
In this paper, nanocomposite hydrogel was synthesized by copolymerization of acrylamide with starch by using potassium persulfate (KPS) as free radical initiator, methylenbisacrylamid (MBA) as cross linker and nano hem...
Determination of mineral chemistry, temperature-pressure gaugeand depth of emplacement of granitoid magmas in the Ardestan region
Felsic intrusions in the studied area are post Eocene and this region is located in the northeast of the Esfahan province. This area belongs to Uremia - daughter magmatic belt in Central Iran. Composition of Felsic intr...