Identification, Isolation and Estimation of flavonoids and effect of growth regulators and salts on flavonoids in Aegle marmelos and Moringa oleifera in vitro
Journal Title: Indian Journal of Scientific Research - Year 2015, Vol 6, Issue 1
Abstract
Unorganized tissues of medicinally useful plants Aegle marmelos and Moringa oleifera were established on MS medium supplemented with 1mg/L BAP+2mg/L 2,4-D and 1.5mg/L BAP +1.5mg/L 2,4-D respectively (standardized MS medium). Parts of established tissues were transferred to standardized (Sd) MS medium fed with various concentrations(1,3,5 mg/L) of growth hormones ( IAA, NAA) and (10,20,30mg/L) salts ( NaCl , KCl ) separately. Tissues at the maximum GI( in all samples) were harvested, dried, powdered and analyzed for estimation of flavonoids. Maximum amount of flavonoids was calculated in callus fed with 2mg/L IAA, NAA and 10mg/L NaCl and KCl in A. marmelos as wll as M. oleifera .
Authors and Affiliations
KAVITA SONI
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