Identification of Candidate Biomarkers and Cancer Genes AHNAK2 and EPPK1 in Pancreatic Cancer
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 18, Issue 8
Abstract
Aims: The lack of specific symptoms at early tumor stages, together with a high biological aggressiveness of the tumor contribute to the high mortality rate for pancreatic cancer (PC). Improved screening for earlier diagnosis, through the detection of diagnostic and prognostic biomarkers provides the best hope of increasing the rate of curatively resectable carcinomas. The aim of this study is to provide new targets for use as biomarkers in PC. Study Design: In a previous study, we identified novel candidate cancer genes and biomarkers that were significantly upregulated in PC, through a meta-analysis of large number of microarray datasets, using bioinformatics methods. In this study, we analyzed the expression of these genes in a panel of pancreatic cancer cell lines by quantitative Reverse Transcription-PCR (qRT-PCR). Place and Duration of Study: Department of Chemistry and Biochemistry and Department of Biology, University of Northern Iowa, USA, between June 2014 and Dec 2015. Methodology: We analyzed the expression of three genes, AHNAK2, EPPK1 and IGHG3 in a panel of seven standard PC cell lines, AsPC-1, BxPC-3, Capan-2, CFPAC-1, HPAF-II, PANC-1, and SW 1990 by Relative Quantification. qRT-PCR experiments were conducted in triplicate, and each experiment was replicated twice using different passages. Results: AHNAK2 was significantly upregulated in all PC cell lines tested, with P values < 0.005 except for PANC-1 (P < 0.05). EPPK1 too was significantly upregulated (P < 0.05) in six of seven PC cell lines tested. While IGHG3 was nominally upregulated in all PC cell lines, upregulation was significant (P < 0.05) in only four PC cell lines. Conclusion: Our results confirm that AHNAK2 and EPPK1 are novel candidates for use as biomarkers in pancreatic cancer. IGHG3 does not appear to be a suitable candidate, due to its low levels of expression in both PC and control cell lines.
Authors and Affiliations
Alex Smith, Logan Poole, Kavita Dhanwada, Nalin C. W. Goonesekere
Keywords
Related Articles
- EP ID EP341084
- DOI 10.9734/BJMMR/2016/28034
- Views 57
- Downloads 0
Epidemiology of Diabetes Mellitus and Molecular Characterization of Bacterial Isolates Recovered from Cases with Asymptomatic Bacteriauria in Trinidad and Tobago
Aims: To highlight the observed features including socio-demographic, economic and biochemical characteristics seen among uncontrolled diabetic adults that should be areas of concern or focus by healthcare providers duri...
Marked Weight Loss, Muscle Wasting and Fatigue on Administration of Empagliflozin in a Subject with Type 2 Diabetes
Background: Weight loss, fatigue and decreased quality of life are frequently reported at the time of initial diagnosis by subjects with Diabetes Mellitus of both types although far more frequently with type 1 and are at...
Laparoscopic Resection of Ileal Gastro Intestinal Stromal Tumor Presenting as Gastro Intestinal Bleed; a Case Report
Gastrointestinal stromal tumors are the rare tumors of GI tract and account for 2% of all the tumors of digestive tract. They are usually found in stomach and are also located in the small intestine. They usually present...
Evaluation of Oral Clonidine Premedication on Intraoperative Blood Loss and Bleeding Severity Score in Functional Endoscopic Sinus Surgery – A Prospective Placebo Controlled Study
Background: Bleeding during functional endoscopic sinus surgery (FESS) remains a challenge for both surgeons and anaesthesiologists despite several modalities available for improving the surgical field. This study was co...
An Investigation of the Retinal Layers in an Experimental Model of Multiple Sclerosis; a Histopathological and Immunohistochemical Study
Background: Animal model of multiple sclerosis is a demyelinating and inflammatory disorder of central nervous system and eye. Histological evaluation in eyes in experimental autoimmune encephalomyelitis (EAE) models dem...