Identification of emerging Chryseobacterium indologenes sepsis using automated identification system, Matrix-assisted Laser Desorption Ionization-time-of-flight Mass Spectrometry (MALDI-TOF) and Pulsed-field Gel Electrophoresis (PFGE) typing
Journal Title: Indian Journal of Microbiology Research - Year 2018, Vol 5, Issue 2
Abstract
Chryseobacterium indologenes is a non-motile, catalase-positive, oxidase-positive, indole-positive, non-glucose-fermenting Gram-negative bacillus that produces yellow coloured colonies on routine culture media. As common environmental saprobe, it is a known colonizer of inanimate objects in the hospital environment. Chryseobacterium indologenes is an emerging .pathogen of nosocomial origin in background of increased use of higher antibiotics. In the present study, positive blood cultures bottled from critically ill patients suspected of nosocomial sepsis were inoculated on routine bacteriological media for a period of six months. The identity and antibiotic sensitivity of Chryseobacterium indologenes were confirmed by Phoenix automated system® followed by automated MALDI-TOF system and Pulsed Field Gel Electrophoresis (PFGE) typing. All the patients had underlying morbidities (7 out of 7 patients). Phoenix automated system and MALDI-TOF system correctly identified the bacterial isolates. We reiterate the fact that levofloxacin should be the first choice of antibiotic in Chyrseobacterium indologens infections followed by cefoperazone + sulbactam. All the isolates were of different clonality as determined by PFGE. To our knowledge, this is the first report on the identification of Chrysobacterium indologenes isolates using automated system, MALDI-TOF and PFGE in India. So, in ICUs of tertiary care centers, where higher antibiotics are used, this bacteria can be an important cause of nosocomial bacteremia.
Authors and Affiliations
Awadhesh Kumar, Chinmoy Sahu, Avinash Singh, Kashi Nath Prasad, Tapan K. N. Dhole
Keywords
Related Articles
- EP ID EP473209
- DOI 10.18231/2394-5478.2018.0060
- Views 35
- Downloads 0
Tube adherence test as a screening tool for detection of biofilm formation among Staphylococcus
Background: Due to large number of infections caused by biofilm producing organism, a reliable and reproducible method for its detection is necessary. Specific methods like Polymerase Chain Reaction (PCR) are available f...
Comparative evaluation of two direct microscopic methods in rapid diagnosis of superficial fungal infection
Introduction: Dermatomycosis is a common fungal infection seen in tropical and subtropical countries. Laboratory diagnosis of Dermatomycosis like dermatophytosis and Pityriasis versicolor depends on the demonstration of...
Emergence of non-albicans Candida species in critical care patients of a tertiary care hospital
Introduction: Candida species are the most common cause of opportunistic fungal infections, resulting in a variety of manifestations ranging from mucocutaneous lesions to life-threatening invasive diseases, particularly...
A retrospective study comprising analysis of CD4 Cell count among HIV infected patients in tertiary care hospital in western India
Introduction: HIV is one of the prevalent infectious disease and major threat to community. As the infection progresses, CD4 count hence, immunity decreases with time. CD4 count plays vital role in prognosis of HIV infec...
Clinico-bacteriological study of pyoderma with special reference to methicillin resistant Staphylococcus aureus
Pyoderma is one of the common skin infections seen in dermatology clinics in India. The changing pattern of etiological agents of pyoderma and the emergence of antibiotic resistant strains are posing a significant proble...