IDENTIFICATION OF ENDEMIC CURCUMA ALBIFLORA THW. FROM CURCUMA SPECIES GROWN IN SRI LANKA BY DNA BARCODING METHOD
Journal Title: International Journal of Pharmacognosy - Year 2018, Vol 5, Issue 4
Abstract
Curcuma is an important genus in traditional medicine. Five plants are reported in Sri Lanka; C. albiflora, C. aromatica, C. longa, C. zedoaria and C. oligantha. Since phylogenetic analysis of gene sequences and combining with complete genomic sequences helps to identify genetic basis of plants, the current study was conducted. Standard CTAB method with little modifications was used for the extraction and purification. Extracted DNA was amplified using universal primers for matK genes in chloroplast genome by PCR (polymerase chain reaction). Sequenced fragments were analyzed and used for DNA barcoding. DNA barcodes {Accession Numbers: C. aromatica (GU180412), C. albiflora (KF 521885), C. longa (JQ409688), C. oligantha (JQ409715), C. zedoaria (JQ409703)} were used for analysis. C. albiflora appeared as a different group as per the Neighbor-Joining method and therefore, it can be identified as a new group. The matK gene of C. albiflora and other species showed, totally 222 variable sites. Therefore, matK gene was an appropriate DNA barcode for identifying C. albiflora from remaining curcuma plants grown in Sri Lanka.
Authors and Affiliations
Thuppahige don Chandima Manjula Kumara Wijayasiriwardene et al.
Keywords
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- EP ID EP431969
- DOI 10.13040/IJPSR.0975-8232.IJP.5(4).223-25
- Views 86
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