IFI16 is Required for an Oligodeoxynucleotide with CCT Repeats to Induce Type I Interferon Production in U937 Cells
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2018, Vol 19, Issue 4
Abstract
In previous study, we found that an oligodeoxynucleotide with CCT repeats (CCT ODN) could display immunosuppressive roles for TLR9 response in mice and in vitro. However, in this study, we found that CCT ODN could obviously elevate the mRNA levels of interferon-inducible protein 16 (IFI16) but no other DNA sensor such as AIM2, which belongs to same family of IFI16, in human monocyte line U937 cells when stimulated for 2 hours. At this condition, CCT ODN could also increase the level of interferon- (IFN-) mRNA in U937 cells. To confirm the CCT ODN-induced IFN- production associated with IFI16, we knocked down IFI16 expression and found that CCT ODN lost its induction role of IFN- production. Then, by using confocal microscope analysis, we found that IFI16 and CCT ODN could co-localize in the cytosol. The cytosol CCT ODN might be delivered by the cell surface DEC-205 that was down regulated after CCT ODN stimulation. Together, the study indicates that CCT ODN can activate IFI16 by associating with DEC-205 to mediate IFN- production in U937 cells, which may be the reason for explaining CCT ODN to attenuate the TLR9 response. The oligodeoxynucleotide with CCT repeats (CCT ODN) is a human microsatellite DNA sequence-based immunosuppressive ODN and displayed selectively negative regulatory role on TLR9 activation [1-3]. It can restrain TLR9 trafficking in immune cells, rescue mice from lethal shock sepsis induced by CpG ODN (TLR9 agonist) and decrease serum anti-DNA antibody levels in mice with lupus-like nephritis with unknown mechanism [4]. As an artificial synthesized single-stranded DNA (ssDNA), CCT ODN might display its role through certain DNA sensor. We know now, in addition to TLR9, there are several DNA sensors, such as DAI, RNA polymerase III, LRRFIP1, AIM2, IFI16 and others, which can sense intracellular DNA to initiate innate immune responses [5-8].
Authors and Affiliations
Shuang Gao, Hong Zhang,, He Li, Tianbo Li, Aobo Du, Xiaoxu Ling, Boqun Cheng, Zhimin Zhang
Keywords
Related Articles
- EP ID EP622664
- DOI 10.26717/BJSTR.2019.19.003357
- Views 151
- Downloads 0
The use of Animals in Assisted Therapy: Systematic Review of the Literature
Animal assisted therapy refers to the use of animals such as dogs, cats, rabbits, turtles, birds, among others in the care of hospitalized patients. This study aimed to analyze the benefits of Dog Therapy for children wi...
Morphological and Technical Factors Associated with Complications and Recanalization of Pericallosal Artery Aneurysms after Endovascular Treatment
We present a series of 31 patients who underwent endovascular treatment due to a pericallosal artery aneurysm. To our knowledge, this is the largest series of patients with pericallosal aneurysms treated by endovascular...
Herbal Extracts Used in Dental Disorders
Natural products have been recently investigated more thoroughly as promising agents for the prevention of oral diseases, especially plaque-related diseases such as dental caries. Oral diseases, a major health issue in t...
Adenosine A2A Receptor Stimulation: A New Strategy for the Treatment of Periodontitis
Periodontitis is an unpleasant clinical condition in which an exaggerated inflammatory reaction occurs in the extravascular gingival tissue. Inflammatory cells release cytokines that amplify tissue inflammation and creat...
Chemical Analysis of Bioactive Compound Produced by Lactobacillusreuteri Isolated From Domestic Chickens in Iran
The purpose was Chemical analysis of the bioactive compounds produced by Lactobacillus reuteri isolated from domestic chickens. In addition, the antimicrobial property of the bioactive compounds was assessed against path...