Immobilization and Kinetic Studies of Bromelain: A Plant Cysteine Protease From Pineapple ( Ananas Comosus) Plant Parts
Journal Title: International Journal of Medical and Health Sciences - Year 2012, Vol 1, Issue 3
Abstract
Bromelain is a cysteine protease enzyme present in the plants. In this study we isolated the enzyme from pineapple plant parts like leaves, green fruit, ripe fruits and immobilized it by using sodium alginate. We observed the effects of different activators and inhibitors, kinetic properties and different concentrations of substrate and estimated enzyme activity on free and immobilized bromelain from pineapple plant parts with hemoglobin as substrate. The protease enzyme showed an effective temperature of 40-60oC and optimal temperature of 50-600C, effective pH of 4.0-8.0 and optimal pH is 4.5-5.5. These all properties of enzymes will be useful in keeping enzyme in an active state in various industrial and medical preparations.
Authors and Affiliations
Sree Padma Priya S, Jayakumar. K, Vijay Mathai, Chintu. S, Sarath Babu. K
Keywords
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