Immune stress up regulates TLR4 and Tollip gene expression in the hypothalamus of ewes
Journal Title: Journal of Animal and Feed Sciences - Year 2013, Vol 22, Issue 1
Abstract
Bacterial endotoxin, LPS, is recognized by Toll-like receptor-4 (TLR4) and induces a signaling cascade leading to synthesis of proinflammatory cytokines and induction of sickness behavior in animals. Transduction of the TLR4 signal is controlled by a potent negative regulator—Toll-interacting protein. The presented study concerns the effect of intravenously injected LPS on the level of expression of TLR4 and Tollip genes in the hypothalamus of ewes. Endotoxin increased (P < 0.01) cortisol release and expression of TLR4 and Tollip genes in the preoptic area (1.87 ± 0.42 and 1.31 ± 0.15), anterior hypothalamus (1.77 ± 0.22 and 1.27 ± 0.13), medial basal hypothalamus (2.53 0.65 and 1.43 ± 0.15), and median eminence (2.93 ± 0.46 and 1.73 ± 0.10), respectively, in comparison with non-treated animals. Our results show that immune stress increases TLR4 gene expression in the hypothalamus. Increased transcription of Tollip may be an attempt to reduce the effect of TLR4 stimulation.
Authors and Affiliations
A. P. Herman, A. Herman, K. Haziak, D. Tomaszewska-Zaremba
Keywords
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