Immunization with Live Promastigote Antigen of Leishmania donovani induces Protection against Experimental Visceral Leishmaniasis Infection in BALB/c Mice.
Journal Title: Journal of Vaccine & Immunotechnology - Year 2015, Vol 2, Issue 1
Abstract
Visceral leishmaniasis (VL) also commonly known as kala-azar is a vector borne parasitic disease which is endemic in Indian subcontinent. At present treatment of this disease relies on chemotherapy and no vaccine is available for human use. Since chemotherapy has some side-effects therefore vaccination with long lasting immunity is the major hope to control leishmaniasis. It is presumed that if a vaccine candidate can activate immune system in a similar manner to the natural course of infection, it will be more successful in generating effective immune response. Also it is known from the previous studies that few numbers of parasites are required to be present in host system for the stimulation of appropriate immune response. Based on this approach we performed our present study in which BALB/c mice were immunized with viable low (103) dose of Leishmania donovani promastigotes. Challenge infection (107) was given to all animals after three weeks of last immunization. Mice were euthanized 15 days after immunization and on 90 post infection/challenge day. A remarkable decline in parasite load was noticed in immunized animals as compared to the infected controls after challenge infection as illustrated by the reduction in parasite burden (72%). Immunized animals showed enhanced DTH responses, elevated IgG2a antibody levels and up-regulated levels of IFN-γ and IL-12 over the infected controls which imply the generation of protective immunity. The present study indicates that such whole parasite vaccines are intrinsically safer and holds a promising potential as anti-leishmanial vaccine candidate.
Authors and Affiliations
Sukhbir Kaur
Keywords
Related Articles
- EP ID EP206085
- DOI 10.13188/2377-6668.1000006
- Views 109
- Downloads 0
Autophagic Control of Listeria Determines the Infection-Induced Death of Macrophages
Listeria induces cell death in macrophages, hepatocytes and dendritic cells. It is accepted that the cell death is induced by listeriolysin O (LLO), which has a crucial role in the escape of Listeria from the vacuole. To...
Signaling Networks Responsible for the Dynamic Modulation of IL-12 in Response to Varying Dosages of LPS in Macrophages
The innate immune response to lipopolysaccharide (LPS) has many possible outcomes depending on the dose, from acute endotoxic shock to broad immunomodulation. High doses (>10 ng/mL) evoke both pro- and anti-inflammatory...
Enhancing Solubilization of Hydrophobic ORF3 Product of Hepatitis E Virus in Bacterial Expression System
Hepatitis E Virus (HEV) is a major cause of acute clinical hepatitis in developing countries. HEV genome has three open reading frames: ORF1, ORF2 and ORF3. ORF3 encodes a small 13 kDa protein (hereinafter called vp13)....
Oral Administration of Lactobacillus pentosus Strain S-PT84 Enhances Anti-Influenza Virus-Specific IgG Production in Plasma after Limited Doses of Influenza Virus Vaccination in Mice
Background: It has been reported that various Lactobacillus species enhance antigen-specific antibody production after viral infection and/or vaccination in animals and humans. In this study, the effect of oral administr...
COBRA-Based Dengue Tetravalent Vaccine Elicits Neutralizing Antibodies Against All Four Dengue Serotypes
Dengue virus (DENV) is the most common arthropod-borne infection in the world. The co-circulation of four serotypes, complex pathogenesis, and potential for antibody-enhanced disease has made vaccine development efforts...