Immunocytochemical staining of cervical smears – A comparative study with routine cytology for confirmation of precancerous and cancerous lesions of cervix
Journal Title: International Journal of Medical Research and Review - Year 2016, Vol 4, Issue 1
Abstract
Introduction: Carcinoma cervix is one of the leading causes of morbidity and mortality. The most effective method of screening is based on Pap smear study. However, Pap smear has relatively low sensitivity and irreducible false negative & false positive rates. In countries like India, it is not always feasible to follow the patient for confirmation by histology. In the present study, we have tried to fill this gap by staining the cervical smears with, epithelial cell markers including CK17 and CEA expressed by dysplastic squamous cells. Materials & Methods: 50 cervical smears reported as precancerous or cancerous were stained with anti CK17 and anti CEA. The patients were followed for histopathology. Results: Out of 20 cases of ASCUS, three were positive both for anti-CK17 and anti-CEA and six were positive for anti- CK17 only. Out of 18 cases of LSIL, nine were positive for both anti-CK17 and anti-CEA and fifteen were positive for anti-CK17 only. Out of eight cases of HSIL, three were positive for both anti-CK17 and anti-CEA and six were positive for anti-CK17 only. Out of 4 cases of SCC, three were positive for both anti-CK17 and anti-CEA and all four were positive for anti-CK17. Conclusion: Positive result in staining was higher in high grade lesions. CK 17 was found to be better marker than CEA. CK17 and CEA can be used as adjunct to Pap smear screening but its high cost may restrict its use in routine.
Authors and Affiliations
Varsha Pandey, Manju Raj Purohit, P. L. Dhand
Keywords
Related Articles
- EP ID EP227457
- DOI 10.17511
- Views 261
- Downloads 0
Factors influencing coronary heart disease mortality: findings from the US National Longitudinal Mortality Study
Introduction: Globally, mortality due to non-communicable diseases (NCDs) is on the rise. Coronary Heart Disease (CHD) is responsible for a large proportion of preventable cardiovascular mortality. This study aims to det...
Comparison of daily and intermittent anti tubercular treatment in achieving sputum negativity in newly diagnosed sputum positive Pulmonary tuberculosis patients
Introduction: Tuberculosis is a major health problem in India. Sputum positive pulmonary tuberculosis patients expel bacilli for a variable period after the start of treatment. The period of infectivity depends on the dr...
Study of outcome after fracture reduction and internal fixation with volar locking plates in distal end radius fractures
Background: The distal end radius fractures are common in older age group patients with osteoporosis. There are many treatment modalities both operative and non-operative methods. In osteoporotic patients the volar locki...
Obstructive sleep apnea and excessive day time sleepiness as measured by epworth sleepiness scale
Introduction: Many patients search for medical care for symptom of a sleep disorder, obstructive sleep apnea hypopnea syndrome (OSAHS). Currently overnight polysomnography is the gold standard for diagnosis of OSAHS. How...
Immunohistochemical expression of Ki-67 in invasive breast carcinoma and its correlation with prognostic parameters
Background: Ki-67 expression is strictly associated with cellular proliferation and is an excellent marker for determining the growth fraction of a given cell population. It may be considered as alternative to mitotic co...