IMMUNOHISTOCHEMICAL STUDY OF CURCUMA XANTORRHIZA ROXB. AND MORINDA CITIRIFOLIA L. ETHANOLIC EXTRACT GRANULES COMBINATION IN HIGH FAT DIET INDUCED HYPERLIPIDEMIA RATS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 11
Abstract
Objectives: These studies were undertaken to assess the mechanism of Curcuma xanthorrhiza and Morinda citrifolia granules combination as anti-hyperlipidemic agent by immunohistochemical analysis in rats model.Methods: The rats were given HFD by oral gavage and PTU in drink water for 10 days to induce hyperlipidemia. Then they were randomized and divided into six groups, negative control, simvastatin control, gemfibrozil control, and three treatment groups. The treatment group rats was treated with extract granules combination at 137; 411; 686 mg/kg body weight that administered orally for 28 days. By immunohistochemistry preparation, jejunum was scored for NPC1L1 expression, so do adipose tissue was score for LPL expression.Results: Curcuma xanthorrhiza and Morinda citrifolia extract granules combination significantly (p<0.05) increased LPL expression at all dose (137; 411; 686 mg/kgBW). Curcuma xanthorrhiza and Morinda citrifolia extract granules combination also significantly (p<0.05) decreased NPC1L1 expression at dose 411 and 686 mg/kgBW, but not significant (p>0.05) at dose 137 mg/kgBW.Conclusions: Curcuma xanthorrhiza and Morinda citrifolia extract granules combination can increase lipoprotein lipase expression, a rate limiting enzyme of plasma triglyceride removal and decrease NPC1L1 expression, an intestinal cholesterol absorption transporter.Â
Authors and Affiliations
Sukardiman . , Suharjono . , Nina Dewi Oktaviyanti
THE STABILITY OF GENERIC MEROPENEM IN TROPICAL COUNTRIES
Objective: To evaluate the stability of two brands of generic meropenem at high ambient temperatures and various concentrations in solution.Methods: Generic meropenem brand A and brand B vials were used to prepare 10 mg/...
STATISTICAL AND KINETIC STUDIES OF ACID PROTEASE BY ASPERGILLUS SPP. ISOLATED FROM SOIL CONTAMINATED WITH ABATTOIR WASTE
Objective: Aim of the present investigation was to optimize the acid protease production from Aspergillus spp. through statistical method in solid state fermentation and to study the inhibitory enzyme kinetics.Methods: T...
SIMPLE HPLC VALIDATED METHOD FOR THE DETERMINATION OF DILTIAZEM HYDROCHLORIDE IN HUMAN PLASMA
Objectives: To develop and validate a high-performance liquid chromatographic method for determination of diltiazem hydrochloride (DLZ) in human plasma.Methods: Mixture of n-hexane and 2-propanol (96:4, ratio) was ad...
ANTIOXIDANT ACTIVITES AND PHYTOCHEMICAL ANALYSIS OF METHANOL EXTRACT OF LEAVES OF ARTOCARPUS HETEROPHYLLUS LAM
Objective: The aim of the present investigation was to evaluate the phytochemical constituents, free radical scavenging activities and antioxidant properties of the methanol extract of leaves of Artocarpus heterophyllus....
PHARMACISTS’ PERCEPTION OF THEIR ROLE AND ASSESSMENT OF CLINICAL PHARMACY EDUCATION TO IMPROVE CLINICAL PHARMACY SERVICES IN INDONESIAN HOSPITALS
Objectives: To identify the pharmacists’ perception on their role, barriers to implement clinical pharmacy services (CPSs), and importance of clinical pharmacy education (CPE) to improve CPSs in Indonesian hospitals.Me...