Immunoinformatics Design of Multi-Epitope Peptide-Based Vaccine Against Cyprinid Herpesvirus-3 (CyHV-3) Targeting Thymidine Kinase Proteins
Journal Title: Journal of Clinical Immunology & Microbiology - Year 2024, Vol 5, Issue 1
Abstract
The common carp Cyprinus carpio is a freshwater teleost and is among the most economically significant fishes in aquaculture throughout the world. Taxonomically, C. carpio are a complex of species including subspecies Cyprinus carpio carpio. C. carpio are now threatened by Cyprinid Herpesvirus-3 (CyHV-3), the causative agent of Koi Herpesvirus Disease (KHVD), which causes severe morbidity and mortality in ornamental koi and common carp and can infect or be transmitted by other species. Despite these devastating circumstances, effective vaccinations or other medications for the control of KHVD are not readily available. For this reason, the aim of the current study was to formulate a multi-epitope vaccine against Cyprinid Herpesvirus-3 (CyHV-3) using an immunoinformatics approach. To assess the immunodominant T- and B-cell epitopes, the CyHV-3 proteomes were employed. Following a thorough evaluation, we constructed a strategy for vaccination employing four possible epitopes selected from among each of the three relevant epitope groups: cytotoxic T-lymphocyte, helper T-lymphocyte and linear B-lymphocyte. Important qualities used in the evaluation of the resultant vaccine are that it will be highly soluble, antigenic, immunogenic and non-allergenic. Among acceptable physicochemical qualities, the anticipated structure of the vaccine bears a close resemblance to that of the original protein. Additional considerations include a robust and sustained predicted binding between the vaccine and the Toll-Like Receptor (TLR9). Simulations of molecular dynamics confirm the likelihood of a strong binding stability and structural tightness. Moreover, the computer-generated immunological simulation revealed that the vaccine, when administered to fish, should induce immune responses comparable to those in real life. Finally, codon optimization based on Escherichia coli K12 produced favorable indications of GC content and acceptably high CAI value, as applicable to the cloning vector pET28+ (a). Overall, these results show that the proposed peptide vaccine is a promising option for CyHV-3 prophylaxis.
Authors and Affiliations
Mohammad Habibur Rahman Molla*, Mohammed Othman Aljahdali, Amer H Asseri, Bushra Jahan, Md Afsar Ahmed Sumon, Nazmul Ahasan Maruf, Khalid Suliman Alsohibany, M Aminur Rahman, Earl T Larson, Nushrat Jahan Maliha, Mohammed Moulay, Christopher L Brown*
Keywords
Related Articles
- EP ID EP733588
- DOI http://dx.doi.org/10.46889/JCIM.2024.5106
- Views 10
- Downloads 0
Characterization, Antifungal Susceptibility and Phenotypic Virulence Markers of Candida Isolates from Blood Stream Infection
Background: Candidemia is a blood stream infection which is caused by various Candida species in children and adults. About more than 200 species of Candida are identified and only about 10% of Candida species cause hu...
Humble Opinions on the Five Very Important Papers
I have the honor to address the 126 critical problems raised by Shanghai Jiao tong University and Chief Editor of Science in celebrating the university’s 126 anniversary [1-5]. My humble opinions and comments are as fo...
Association between Non-Right-Handedness and Multiple Sclerosis (MS)
Background: Only two studies have examined the relationship between handedness and Multiple Sclerosis (MS). Objective: To extend previous work examining any relationships between MS and handedness. Methods: Using a...
Explaining the Cancer Cure with Frequencies
I spent 40 years of my life investigating the human aura. First, I started photographic it - it can be photographed in high frequency electric field and it is called Kirlian photography. I found that positive emotions...
Bias in Science Arbitration: An Authorship Viewpoint
The objective of this article was to discuss the potential bias in arbitration of unknown researchers’ scientific work in reputable journals. Publishing an article in a quality international journal is a dream that globa...