Impact of HLA antigen and antibody expression on cross-matching and transfusion effect
Journal Title: Chinese Journal of Blood Transfusion - Year 2025, Vol 38, Issue 3
Abstract
[Objective] To study the relationship between the expression intensity of HLA-Ⅰ platelet antibodies in patients with platelet transfusion refractoriness (PTR) and platelet cross-matching, and to further evaluate other factors in order to provide relevant data support for improving platelet transfusion efficiency and optimizing platelet transfusion regimens. [Methods] Luminex single antigen flow cytometry was used to detect platelet specific antibodies in 35 patients with hematological disease. Subsequently, the Capture-P method was employed to perform 102 crossmatchings between plasma with HLA-Ⅰ antibodies and platelets with known HLA-Ⅰ genotypes. The cross-matching results were assessed and the clinical transfusion outcomes were tracked. [Results] The positive detection rate of HLA-Ⅰ and HPA antibodies in this study was 48.6% (17/35). The negative rate of cross-matching in 102 cases was 37.3% (38/102). Multiple factors affect platelet cross-matching, such as HLA-Ⅰ antibody expression level and antibody type, antigen expression level, cross-reactivity group and eplets. Among them, the expression level and antibody type of HLA-Ⅰ antibody are the main influencing factors. However, the effectiveness of clinical platelet transfusion is not completely determined by the compatibility of platelet cross-matching. [Conclusion] In addition to avoiding strong positive HLA-Ⅰ antibodies, clinical matching should also be vigilant against the serological cross-incompatibility caused by weak positive HLA-Ⅰ antibodies. It may be necessary to establish HLA-Ⅰ low expression antigen database as a better alternative platelet donor selection strategy, and gradually explore the effectiveness of ‘low expression mismatch’ strategy for clinical platelet transfusion.
Authors and Affiliations
Honghong HE, Jing CHENG, Yihan WANG, Min JIANG, Longhai TANG
Keywords
Related Articles
- EP ID EP763273
- DOI 10.13303/j.cjbt.issn.1004-549x.2025.03.003
- Views 23
- Downloads 0
Efficacy of apheresis and concentrated platelets under different transfusion strategies
Objective To retrospectively analyze the clinical effects of apheresis and concentrated platelets under different transfusion strategies, so as to explore more scientific transfusion strategies. Methods A total of 279 pa...
Preparation and in vitro evaluation of platelet membrane biomimetic liposomes loaded with vincristine sulfate
[Objective] To prepare platelet membrane biomimetic liposomes loaded with vincristine sulfate (VCR) for targeted delivery to tumor. [Methods] Vincristine sulfate liposomes (LIPO) were prepared using the pH-gradient metho...
Therapeutic effect of autologous platelet-rich plasma on chronic endometritis
[Objective] To evaluate the therapeutic effect of autologous platelet-rich plasma (PRP) for chronic endometritis (CE). [Methods] A retrospective analysis was conducted on 36 patients with CE who had failed antibiotic tre...
Analysis of pathogenic microorganisms in 64 077 cases of umbilical cord blood
Objective To investigate the detection of pathogenic microorganisms in umbilical cord blood and maternal blood from 2012 to 2021, so as to improve the collection of umbilical cord blood and guarantee the safety of umbili...
Retrospective analysis of quality data of different blood components in Chongqing
Objective To provide reference for formulating relatively unified quality control strategies and meeting the requirements of homogenization construction of blood banks across Chongqing area by retrospectively analyzing s...