Impact of Particle-Size Reduction on the Solubility and Antidiabetic Activity of Extracts of Leaves of Vinca rosea
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2019, Vol 16, Issue 3
Abstract
Objectives: The present study aimed to enhance the aqueous solubility of methanol extract of leaves of Vinca rosea (family: Apocynaceae) by particle-size reduction using milling and to evaluate its antidiabetic activity. Materials and Methods: The methanol extract (ME) was micronized using a vibratory ball mill, operated at a vibratory speed of 15 Hz for 60 min at room temperature, and the resulting extract micronized ME (MME) was investigated to determine particle size, solubility, UV/visible profile, and in vitro antidiabetic activity. Results: The average particle size of MME was 0.753±0.227 μm, which was less than half of that of the ME (2.007±0.965 μm). The solubility of MME was greater than that of the ME. MME exhibited 65.63%, 18.0%, and 96.87% higher antidiabetic activity in the glucose uptake by the yeast cells method, hemoglobin glycosylation assay, and the alpha amylase inhibition assay, respectively (p<0.05). Conclusion: The results of the present study indicate that micronization effectively enhanced the aqueous solubility and antidiabetic activity of methanol extract of leaves of Vinca rosea.
Authors and Affiliations
Khalid HUSSAIN, Abida QAMAR, Nadeem Irfan BUKHARI, Amjad HUSSAIN, Naureen SHEHZADI, Shaista QAMAR, Sajida PARVEEN
Keywords
Related Articles
- EP ID EP48830
- DOI 10.4274/tjps.galenos.2018.02419
- Views 268
- Downloads 0
Design and In Vitro Evaluation of Eudragit-Based Extended Release Diltiazem Microspheres for Once- and Twice-Daily Administration: The Effect of Coating on Drug Release Behavior
Objectives: The aim of this investigation was to develop an extended release formulation of diltiazem hydrochloride (DL) for once- and twice-daily administration, based on Eudragit (Eud) RL and RS microspheres using emul...
Global DNA Hypomethylation and Rassf1a and c-myc Promoter Hypermethylation in Rat Kidney Cells after Bisphenol A Exposure
Objectives: Bisphenol A (BPA) is a synthetic monomer used in the production of polycarbonate and an environmental contaminant with endocrine disrupting properties. BPA release from plastic carriers is thought to cause hi...
Masticatory Functional Load Increases the mRNA Expression Levels of ACTN2 and ACTN3 and the Protein Expression of α-Actinin-2 in Rat Masseter Muscle
Objectives: α-actinins play structural and regulatory roles in cytoskeletal organization. They form a lattice structure that secures actin in thin filaments, which generate and transmit muscle contractile forces. The mor...
QSAR Studies on Neuraminidase Inhibitors as Anti-influenza Agents
Objectives: The present study aimed to establish significant and validated quantitative structure-activity relationship (QSAR) models for neuraminidase inhibitors and correlate their physicochemical, steric, and electros...
An In Vitro Study on the Interactions of Pycnogenol® with Cisplatin in Human Cervical Cancer Cells
Objectives: In the treatment of cancer, it is intended to increase the anticancer effect and decrease cytotoxicity using various plant-derived phenolic compounds with chemotherapeutic drugs. Pycnogenol® (PYC), a phenolic...