IMPROVED RAT STROKE MODEL BY INTRALUMINAL MIDDLE CEREBRAL ARTERY OCCLUSION: A SPECIAL EMPHASIS ON SURGICAL TECHNIQUE
Journal Title: Health Problems of Civilization - Year 2017, Vol 11, Issue 3
Abstract
Various rodent models have been developed for examining either focal or global cerebral ischemia so far, but the most used model is the rat middle cerebral artery occlusion by intraluminal filaments, followed by reperfusion, and 2,3,5-triphenyltetrazoliumchloride or Cresyl Violet/Nissl staining for evaluation of the region of interest. Like other surgical procedures, this one is prone to various complications (excessive bleeding, vascular or nerve lesions) and failure related to the surgical technique. We focused on detailed surgical techniques, along with data from literature, in order to reduce the complications and increase the chance of experiment success. Magnetic Resonance Imaging evaluation with 3D reconstruction of the ischemic area opens new perspectives on investigations into the ischemic brain, having the advantage of being noninvasive as an alternative to gold histological standard for measuring the infarct size, being the only one accesible in surviving subjects.
Authors and Affiliations
Alex Orădan, Adina Huțanu, Emőke Horváth, Liviu Chiriac, Minodora Dobreanu
Keywords
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- EP ID EP321698
- DOI 10.5114/hpc.2017.70008
- Views 62
- Downloads 0
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