Improvement of pulsed electromagnetic fields on bone mass and microstructure in rats
Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 6
Abstract
Objective To investigate the changes of bone mass and microstructure in rats exposed to pulsed electromagnetic fields (PEMF), and to clarify the effects of PEMF on bone improvement in rats. Methods Sixteen 12-week-old SD rats were randomly divided into two groups, with 8 rats in each group. The rats without PEMF stimulation were in the normal group, and those with PEMF stimulation were in the PEMF group. Rats in the PEMF group were placed in the center of the coils of the device to receive PEMF stimulation (2 mT, 15 Hz) with 6 h / d for 6 weeks. Rats in the control group were also placed in the coils without PEMF stimulation. The body mass and food intake of rats were observed and recorded continuously during the experiment. The number of vertical or horizontal behavioral activities of rats was monitored by open field test. Micro-CT was used to scan trabecular and cortical bone of femoral metaphysis in rats, and relevant parameters of femoral bone mass and microstructure were analyzed, including bone mineral density (BMD), bone volume per total volume (BV / TV), trabecular thickness ( Tb. Th), trabecular number ( Tb. N), trabecular separation ( Tb. Sp) and cortical thickness (Ct. Th). Results No significant difference in body mass and food intake was observed between the normal group and PEMF group during the experiment (P > 0. 05). The results of open field test showed that PEMF stimulation exerted no significant effect on the vertical or horizontal behavioral activities of rats ( P > 0. 05) . Micro-CT analysis revealed that BV / TV, BMD, Tb. N, Tb. Th and Ct. Th of PEMF group were significantly higher than those of normal group (P < 0. 01), and Tb. Sp was significantly lower than that of normal group (P < 0. 01). Conclusion PEMF stimulation can significantly improve the bone mass and microstructure of rats without affecting the food intake, body mass and locomotor activity, and has a significant effect on bone development.
Authors and Affiliations
AN Dike, WANG Jingquan, WANG Dan, SHAO Xi, LIU Juan, YAN Zedong, HUANG Jinghui, LUO Zhuojing,JING Da
Keywords
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