Improvement of Regeneration of Pelargonium radula via Somatic Embryogenesis
Journal Title: Biotechnology Journal International - Year 2015, Vol 5, Issue 4
Abstract
In vitro stem segments of Pelargonium radula cultured for callusing then differentiated into somatic embryos and subsequently regenerated plantlets. Initiation of callus was observed in culture medium containing low concentrations of the plant growth regulators, 2,4-dichlorophenoxyacetic acid (2,4-D) and/or α-naphthalene acetic acid (NAA). At 0.2 mg/L 2,4-D and 0.2 mg/L NAA was showing the highest rate (92%) of callus induction. The callus showed no sign of browning after sub-cultured. Sub-culturing the callus onto medium with 0.2 mg/L 2,4-D showed the highest in proliferation rate resulted 13.45g weight of callus. The presence of agar at 6 g/L and 0.5 mg/L Gibberellic acid (GA3) improved the regeneration of the somatic embryos, which produced maximum number of plantlets (15 plantlets). Agar with concentration of 9 to12 g/L increased the incidence of browning. The former medium was more successful in plantlet regeneration when the selected embryos were subsequently transferred to regeneration medium with 3 g/L agar, 0.5 mg/L GA3 and 0.5 mg/L Benzylaminopurine (BAP).
Authors and Affiliations
A. R. Zuraida, M. A. Mohd Shukri, O. Ayu Nazreena
Keywords
Related Articles
- EP ID EP237090
- DOI 10.9734/BBJ/2015/15337
- Views 133
- Downloads 0
Improvement of Regeneration of Pelargonium radula via Somatic Embryogenesis
In vitro stem segments of Pelargonium radula cultured for callusing then differentiated into somatic embryos and subsequently regenerated plantlets. Initiation of callus was observed in culture medium containing low conc...
Influence of Dominant Follicle and Corpus luteum on Recovery of Good Quality Oocytes for In vitro Embryo Production in Cattle
The quality of oocytes is more critical for the success of bovine in vitro embryo production (IVP) than the conditions of in vitro culture of these structures. Despite knowledge about bovine follicular development, espec...
Antibacterial Activities of Six Medicinal Plants Used Traditionally by Saudi People to Treat Common Diseases
Antibiotic resistance is increasing and dangerous problem resulting in a decrease in the used number and fully active antimicrobial agents available to treat infections with multi-drug resistant (MDR) bacteria. Herbal me...
Plant Regeneration Studies in Euphorbia fusiformis through Somatic Embryo Genesis
Euphorbia fusiformis is a rare medicinal plant. The genus Euphorbia belongs to the family Euphorbiaceae. E. fusiformis is commercially useful for production of latex and has many medicinal values. Based on the importance...
Comparative Occurrence of Resident Fungi on Gamma Irradiated and Steam Sterilized Sorghum Grains (Sorghum bicolor L.) for Spawn Production in Ghana
Sorghum is one of the important cereals consumed by humans, animals and also used for the production of mushroom spawns in Ghana. Aim: Identification of fungi present on sorghum grains before and after pretreatment (ste...