Improving the Technological Cycle of Microclonal Propagation of Rubus chamaemorus L.
Journal Title: Lesnoy Zhurnal (Russian Forestry Journal) - Year 2024, Vol 5, Issue 5
Abstract
The results of the study of the microclonal propagation of the cloudberry (Rubus chamaemorus L.) of the Leningradskaya and Kondinskaya forms at the stages of microclonal propagation itself and rooting of microshoots in in vitro culture are presented. R. chamaemorus is one of the most popular bog berry plants in the countries of Northern Europe and the northern regions of Russia, possessing highly valuable nutritional and pharmacological properties. In order to intensify industrial berry growing in Russia and meet the market demand for berry products in the context of import substitution, it is necessary to use high-tech methods for obtaining planting material. In order to preserve the valuable gene pool and accelerate the production of a large amount of healthy planting material of R. chamaemorus forms, it is necessary to improve and optimize the technologies or microclonal propagation of this species. The largest number (on average 9.6â9.9 pcs.) and the total length (16.4â 19.5 cm) of R. chamaemorus microshoots in in vitro culture at the stage of microclonal propagation itself have been observed on the Murashige and Skoog culture medium. An increase in the concentration of the âDroppâ preparation from 0.1 to 0.2 mg/l in the culture medium has contributed to an increase in the number of R. chamaemorus microshoots (on average by 1.8â2.4 times), an increase in their total length in the Konsinskaya form (by 1.5 times) and its decrease in the Leningradskaya form (by 1.1 times). The largest number (on average 3.9â4.6 pcs.) and the total length (13.2â14.0 cm) of R. chamaemorus roots at the stage of microshoot rooting in vitro have been noted on the Murashige and Skoog culture medium. An increase in the concentration of indolebutyric acid from 0.5 to 1.0 mg/l in the culture medium has contributed to an increase in the number of R. chamaemorus roots (on average by 1.4 times) and a decrease in their total length (by 1.15â1.25 times).
Authors and Affiliations
Aleksandr M. Antonov, Anton I. Chudetsky, Yuliya S. Cheryatova, Irina B. Kuznetsova, Elena I. Kulikova
Keywords
Related Articles
- EP ID EP750040
- DOI 10.37482/0536-1036-2024-5-214-226
- Views 7
- Downloads 0
Forestry Measures in Shelterbelts of the Krasnodar Territory
In the process of growth and development of forest belts, some of their taxation characteristics and design change. Forest reclamation complexes are subject to homeostasis. Changing the design of shelterbelts leads to cr...
Processing Factors and Properties of Thermal Insulation Boards Made of Plant Fillers
Wood processing soft waste is mainly used in the production of fuel briquettes, irrecoverable (non-recyclable) waste from spinning flax and cotton are incinerated or sent to dump. The development of methods for recycling...
Influence of Growth Stimulants on Seed Germination and Seedlings Growth of Scots Pine (Pinus sylvestris L.)
Scots pine (Pinus sylvestris L.) is a potential introduced plant that can be used for reforestation in the southern part of Primorsky Krai. This plant is valuable for water regulation and mountain strengthening. It is wi...
The Current State of Conifer Species Breeding and Seed Production
The article analyzes and summarizes information on breeding and seed production of the main forest-forming and commercial species for the countries of Northern Europe and Russia: Scots pine (Pinus sylvestris L.) and Norw...
The Conservation of Plant Species Diversity in Forest Management in the Key Biotopes of the Vologda Region
A reduction in the number of biological species can cause a loss of the integrity of the biosphere and its ability to maintain the most important characteristics of the natural environment. Therefore, scientific research...