In vitro Activities of Antimicrobial Agents against Uropathogenic Isolates at Brong Ahafo Regional Hospital, Ghana
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2017, Vol 6, Issue 5
Abstract
Bacterial resistance against antimcrobial agents is a growing international problem in the treatment of nosocomial infections, especially in developing countries. This study therefore sought to determine the antibiotic susceptibility pattern of the isolates in UTIs and to identify the probable antibiotic alternatives in uropathogenic infection to guide antimicrobial therapy at the Brong-Ahafo Regional Hospital-Sunyani. Between January and December 2014, 200 urinary isolates were received from in and out patients at the Microbiology unit of the laboratory. Isolates were tested for antimicrobial susceptibility by the Kirby Bauer disc diffusion method, on Mueller-Hinton Agar (Oxoid GmbH, Wesel, Germany) and the results were presented as resistant or susceptible, according to the recommendations of Clinical and Laboratory Standards Institute (CLSI). One hundred and seventy-two (172, 96.6%) isolates were resistant to tetracycline and 144(90.5%) isolates were resistant to Ampicillin/sulbactam. One hundred and fifty (150, 93.2%) isolates were readily susceptible to Amikacin. Among the third generation cephalosporins, ceftizoxime achieved 50% sensitivity and 20.6% and 16.7% for cefotaxime and ceftazidime respectively against all isolates. The isolates also showed strong resistance to the fluoroquinolones, nalidixic acid (80.8%); ciprofloxacin (74.1%); ofloxacin (65.4%) and levofloxacin (64.6%). Chloramphenicol and gentamicin achieved 23.4% and 24.1% sensitivity respectively. Lower resistance was observed in amikacin and ceftizoxime. The extent of resistance among bacteria isolates in UTIs in non-hospitalized and hospitalized patients is worryingly high in the Brong-Ahafo Regional Hospital. Antimicrobials such as tetracycline, ampicillin/sulbactam, chloramphinicol and gentamicin should no longer be recommended for initial empirical therapies for UTIs especially when E. coli is concerned. Amikacin, ceftizoxime, ofloxacin and levofloxacin may be considered as alternatives.
Authors and Affiliations
Solomon Wireko, Amina Abubakari, Bempah Opoku
Keywords
Related Articles
- EP ID EP570709
- DOI 10.20546/ijcmas.2017.605.023
- Views 58
- Downloads 0
Deproteinated Cheese Whey Medium for Biomass Production of Probiotic Lactobacillus helveticus MTCC 5463
Deproteinated cheese whey was used for biomass production of probiotic strain Lactobacillus helveticus MTCC 5463 in a biofermenter. Optimization of growth parameters such as temperature, pH and time of incubation as well...
Standardization of Tinting Techniques in China aster cv. Local White
Value addition in floriculture increases the economic value and consumer appeal of any floral commodity. The profitability of a commodity is increased when the raw material is converted into a value added product which c...
Occurrence of Fungal Diseases and Their Importance on Date Palm in Sudan
Date palm (Phoenix dactylifera L.) is an important fruit tree in north of Sudan and it was observed to be severely affected by many diseases. This study was carried out to evaluate the incidence and distribution of funga...
Diversity of Tomato Leaf Curl Virus (Tolcv), Bemisia tabaci and its Transmission
Tomato leaf curl virus (ToLCV) is a very important pathogen in the tomato grown belt of India and world as a whole. It persist wide diversity and many isolate has been identified however molecular marker has represented...
Efficacy of Organically Managed Cropping System in Improvement of Soil Health in Ne Hill Region
Field experiments were conducted during the kharif seasons of 2015 and 2016 to evaluate the efficacy of the organically managed cropping system in improvement of soil health in NE hill region. Four combinations of two cr...