In vitro Activities of Antimicrobial Agents against Uropathogenic Isolates at Brong Ahafo Regional Hospital, Ghana
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2017, Vol 6, Issue 5
Abstract
Bacterial resistance against antimcrobial agents is a growing international problem in the treatment of nosocomial infections, especially in developing countries. This study therefore sought to determine the antibiotic susceptibility pattern of the isolates in UTIs and to identify the probable antibiotic alternatives in uropathogenic infection to guide antimicrobial therapy at the Brong-Ahafo Regional Hospital-Sunyani. Between January and December 2014, 200 urinary isolates were received from in and out patients at the Microbiology unit of the laboratory. Isolates were tested for antimicrobial susceptibility by the Kirby Bauer disc diffusion method, on Mueller-Hinton Agar (Oxoid GmbH, Wesel, Germany) and the results were presented as resistant or susceptible, according to the recommendations of Clinical and Laboratory Standards Institute (CLSI). One hundred and seventy-two (172, 96.6%) isolates were resistant to tetracycline and 144(90.5%) isolates were resistant to Ampicillin/sulbactam. One hundred and fifty (150, 93.2%) isolates were readily susceptible to Amikacin. Among the third generation cephalosporins, ceftizoxime achieved 50% sensitivity and 20.6% and 16.7% for cefotaxime and ceftazidime respectively against all isolates. The isolates also showed strong resistance to the fluoroquinolones, nalidixic acid (80.8%); ciprofloxacin (74.1%); ofloxacin (65.4%) and levofloxacin (64.6%). Chloramphenicol and gentamicin achieved 23.4% and 24.1% sensitivity respectively. Lower resistance was observed in amikacin and ceftizoxime. The extent of resistance among bacteria isolates in UTIs in non-hospitalized and hospitalized patients is worryingly high in the Brong-Ahafo Regional Hospital. Antimicrobials such as tetracycline, ampicillin/sulbactam, chloramphinicol and gentamicin should no longer be recommended for initial empirical therapies for UTIs especially when E. coli is concerned. Amikacin, ceftizoxime, ofloxacin and levofloxacin may be considered as alternatives.
Authors and Affiliations
Solomon Wireko, Amina Abubakari, Bempah Opoku
Keywords
Related Articles
- EP ID EP570709
- DOI 10.20546/ijcmas.2017.605.023
- Views 53
- Downloads 0
Studies of Genetic Variability, Heritability and Genetic Gain for Some Important Horticultural Traits in Cauliflower (Brassica oleracea var. botrytis L.)
Twenty mid-late and late group cauliflower genotypes were evaluated during Rabi season of 2016 at the Experimental Farm of the Department of Vegetable Science, Dr. YS Parmar University of Horticulture and Forestry, Solan...
Regulation of Phytosiderophore (PS) and Yellow Stripe-1 (YS1) Transporter Activity by Sulphur (S) and that of High-Affinity Sulphate (SULTR1; 1) Transporter by Iron (Fe) in Wheat
Deficiency of micronutrients in soil particularly, that of Fe is a major nutritional and production constraint worldwide. We hypothesize a role of sulphur nutrition in altering the Fe deficiency tolerance response of cro...
Plasmid Mediated Quinolone Resistance Determinants among Nosocomial Clinical Pseudomonas aeruginosa Isolates
Quinolones are among the most commonly utilized antimicrobials for treatment of infections caused by P. aeruginosa. Plasmid mediated quinolone resistance (PMQR) is an expanding clinical concern worldwide with fluctuating...
Genetic Divergence in Wheat (Triticum aestivum L.Thell.) Under Saline Sodic Condition
Recently, abiotic stress like salinity, drought and temperature are the severe problems to worldwide wheat production, mostly in arid and semiarid areas. A feasible solution is breeding for salt-tolerant cultivars of whe...
Non-Dairy Probiotics – An Emerging Trend in Health Care Products
The beneficial effects of consuming probiotics wouldn’t have been possible without the extensive research for the past two decades. However, due to enhanced awareness and health consciousness among today’s population, th...