In-vitro alpha amylase and alpha glucosidase inhibitory potential of leaf hexane, ethyl acetate and methanol fractions of Pterocarpus soyauxii Taub
Journal Title: Trends in Phytochemical Research - Year 2020, Vol 4, Issue 1
Abstract
Diabetes mellitus is a critical clinical condition characterized by hyperglycemia in which an accelerated amount of glucose circulates in the blood plasma. This work attempts to evaluate the α-amylase and α-glucosidase inhibitory activities of hexane, ethyl acetate and methanol fractions of Pterocarpus soyauxii at varying concentrations. 1.0 kg of milled leaf sample was first soaked in 5 L of methanol to obtain a percentage yield of 12%. The assay was carried out using standard procedures. The following IC50 values were obtained in the α-amylase inhibition: 0.0395, 0.05995, and 0.0509 mg/mL for hexane, ethyl acetate, and methanol, respectively compared to the standard drug acarbos (0.00812 mg/mL). A dose-dependent increase in percentage inhibition was obtained for α-glucosidase with IC50 values of 0.052, 0.059, and 0.065 mg/mL for respective fractions compared to the standard drug used (0.0017 mg/mL). The hexane fraction showed the greatest percentage inhibition for both α-amylase and α-glucosidase, while appreciable inhibition activity was observed in other fractions. The Nigerian Pterocarpus soyauxii has been identified for the first time as a very potent anti-diabetic agent useful in management of postprandial hyperglycemia and related therapeutic interventions.
Authors and Affiliations
Emmanuel Ojah; Dorcas Olufunke Moronkola
Veronica crista-galli Steven and Veronica persica Poir. as anticancer and antioxidant plants in-vitro
The antioxidant activities and reducing power capabilities of Veronica crista-galli and Veronica persica were evaluated using DPPH radical scavenging assay, total antioxidant activity and MTT assay at incubation times of...
Antioxidant activity of Allanblackia floribunda trunk bark ethanol extract on alcohol-induced hypertensive rats
Oxidative stress is involved in many chronic diseases. The ethanol extract of Allanblackia floribunda (A. floribunda) was investigated for the antioxidant and antihypertensive activities on alcohol hypertensive rat’s mod...
The rhizome essential oil of Curcuma aeruginosa Roxb. (Zingiberaceae) from Vietnam
The volatile compounds identified from the rhizome oil of Curcuma aeruginosa Roxb (Zingiberaceae) collected from Cham Chu Natural Reserve, Tuyen Quang Province, Vietnam, are reported. The plant sample was air-dried for t...
Automated structure elucidation of phytochemicals
Plants produce a variety of chemical compounds, and plants have been the main source of new chemical entities and novel chemical scaffolds or templates, unfolding new challenges for organic synthetic chemists to explore...
Quantification of ursolic acid, correlations and contribution by other traits towards accumulation of ursolic acid in six Ocimum species
Sixteen accessions belonging to six species of Ocimum from Uttar Pradesh (12), Andhra Predesh (3) and Marashtra (1) states of India were screened for ursolic acid and higher oil yields. A considerable amount of genetic v...