In vitro alpha-glucosidase inhibitory activity of phenolic constituents from aerial parts of Polygonum hyrcanicum
Journal Title: DARU Journal of Pharmaceutical Sciences - Year 2012, Vol 20, Issue 0
Abstract
Background and the purpose of the study The early stage of diabetes mellitus type 2 is associated with postprandial hyperglycemia. Hyperglycemia is believed to increase the production of free radicals and reactive oxygen species, leading to oxidative tissue damage. In an effort of identifying herbal drugs which may become useful in the prevention or mitigation of diabetes, biochemical activities of Polygonum hyrcanicum and its constituents were studied. Methods Hexane, ethylacetate and methanol extracts of P. hyrcanicum were tested for alpha-glucosidase inhibitory, antioxidant and radical scavenging properties. Active constituents were isolated and identified from the methanolic extract in an activity guided approach. Results A methanolic extract from flowering aerial parts of the plant showed notable alpha-glucosidase inhibitory activity (IC50 = 15 mug/ml). Thirteen phenolic compounds involving a cinnamoylphenethyl amide, two flavans, and ten flavonols and flavonol 3-O-glycosides were subsequently isolated from the extract. All constituents showed inhibitory activities while compounds 3, 8 and 11 (IC50 = 0.3, 1.0, and 0.6 muM, respectively) were the most potent ones. The methanol extract also showed antioxidant activities in DPPH (IC50 = 76 mug/ml) and FRAP assays (1.4 mmol ferrous ion equivalent/g extract). A total phenol content of 130 mg/g of the extract was determined by Folin-Ciocalteu reagent. Conclusion This study shows that P. hyrcanicum contains phenolic compounds with in vitro activity that can be useful in the context of preventing or mitigating cellular damages linked to diabetic conditions.
Authors and Affiliations
F Moradi-Afrapoli, M Mirjani, Abbas Hadjiakhoondi, Narguess Yassa, Peyman Salehi, Behavar Asghari, Mobina Malmir, Soodabeh Saeidnia, Yusef Ajani, Reza Dolatabadi, Mattias Hamburger
Keywords
Related Articles
- EP ID EP140530
- DOI -
- Views 105
- Downloads 0
Perspective on lead toxicity, a comparison between the United States and Iran
Lead is a pervasive toxin that has been implicated in human poisonings throughout history. Exposure mitigation strategies in the United States and worldwide have led to a decline in symptomatic poisonings and population...
Structural relationships and vasorelaxant activity of monoterpenes
Background and purpose of the study The hypotensive activity of the essential oil of Mentha x villosa and its main constituent, the monoterpene rotundifolone, have been reported. Therefore, our objective was to evaluate...
A systematic review on status of lead pollution and toxicity in Iran; Guidance for preventive measures
Lead is an old environmental metal which is presented everywhere and lead poisoning is an important health issue in many countries in the world including Iran. It is known as a silent environmental disease which can have...
Single course of high dose dexamethasone is more effective than conventional prednisolone therapy in the treatment of primary newly diagnosed immune thrombocytopenia
IntroductionImmune thrombocytopenia (ITP) is an immune disorder commonly presents as isolated thrombocytopenia. Generally corticosteroids are the main treatment of ITP. This study was designed to evaluate effectiveness o...
Investigation on in vitro dissolution rate enhancement of indomethacin by using a novel carrier sucrose fatty acid ester
Background and the purpose of the study The purpose of the present investigation was to characterize and evaluate solid dispersions (SD) of indomethacin by using a novel carrier sucrose fatty acid ester (SFE 1815) to in...