IN VITRO AND IN SILICO EVALUATION OF THE ANTIDIABETIC EFFECT OF HYDROALCOHOLIC LEAF EXTRACT OF CENTELLA ASIATICA
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 8
Abstract
Objective: Aim of the present study was to explore the possible mechanisms through which Centella asiatica may be beneficial in managing diabetes and its associated complications by in vitro methods and to predict the potential bioactive constituent/s responsible for its anti-diabetic activity through in silico docking study.Methods: Hydro-alcoholic leaf extract of C. asiatica was prepared using Soxhlet extraction. Plant extract was evaluated for its in vitro antioxidant, anti-inflammatory and anti-diabetic activity. Further, docking screening was performed using Molegro Virtual Docker software to predict potential moiety which may be responsible for its anti-diabetic activity.Results: Soxhlet extraction resulted in extractive yield of 35.43% and showed high antioxidant potential as demonstrated by its ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (98.72%). This antioxidant activity may be attributed to high phenolic and flavonoid components present in extract (1.004 mg gallic acid equivalent (GAE)/gram and 0.113 mg Rutin equivalent (RE)/gram dried extract respectively). Plant extract inhibited albumin denaturation (81.77%) and stabilized RBCs membrane (66.7%) indicating its high anti-inflammatory potential. In vitro anti-diabetic assays revealed that anti-hyperglycaemic activity of this plant can be attributed to its high efficiency to inhibit α-amylase (62.13%) and glucosidase (59.9%) enzymatic activity, which are well established targets for the management of diabetes. Further, through docking studies we predicted that centellasaponin-C, asiaticoside, asiaticoside-E, castilliferol and brahminoside present in this plant might be responsible for the anti-diabetic properties exhibited by this plant.Conclusion: These results provide a scientific justification for the traditional anti-diabetic use of this plant. It may control diabetes through lowering dietary glucose uptake and may benefit in progression of diabetic complications through reducing oxidative and inflammatory stress. Predicted anti-diabetic molecules need to be screened further for the management of hyperglycemia.
Authors and Affiliations
Vineet Mehta, Arun Sharma, Shruchi Tanwar, Udayabanu Malairaman
Keywords
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