In vitro and in vivo activities of ticarcillin-loaded nanoliposomes with different surface charges against pseudomonas aeruginosa (ATCC 29248)
Journal Title: DARU Journal of Pharmaceutical Sciences - Year 2012, Vol 20, Issue 0
Abstract
Pseudomonas aeruginosa exhibits multiple antibiotic resistance mechanisms. Different studies have shown that entrapment of antibiotics into liposomes could increase their anti- Pseudomonas activity. The objectives of this study were to prepare ticarcillin loadednanoliposomes with variable surface charges and evaluate their in vitro and in vivo efficacies against Pseudomonas aeruginosa (ATCC 29248). Methods Ticarcillin-loaded nanoliposomes with positive, negative and neutral surface charges were prepared by extrusion method. Ticarcillin encapsulation efficacies for different formulations were measured by HPLC method. Minimum inhibitory concentration (MIC) of ticarcillin nanoliposomal forms against strain ATCC 29248 were determined by broth dilution method. The killing rate of Pseudomonas aeruginosa was exposed to various concentrations of ticarcillin in free and nanoliposomal forms were analyzed. Ultimately, in vivo therapeutic efficacy of nanoliposomes in burned mice skin infected with strain ATCC 29248 was investigated.Results The encapsulation efficacies for ticarcillin-loaded cationic nanoliposomes were significantly higher (76%+/-0.17) than those of neutral (55%+/-0.14) and anionic (43%+/-0.14) nanoliposomes. The MIC of free, cationic, neutral and anionic nanoliposomal forms of ticarcillin against ATCC 29248 were to 24, 3, 6 and 48 mg/L, respectively. The killing rates of ticarcillinloaded cationic nanoliposomes were higher than those of free and other drug formulations. Treatment by ticarcillin-loaded nanoliposomes with positive, neutral and negative surface charges resulted in almost 100, 60 and 20% survival rates, respectively. Conclusion Our data suggested that cationic ticarcillin-loaded nanoliposomes because of high effectiveness would be a good choice to treatment of Pseudomonas aeruginosa infections
Authors and Affiliations
Amir Gharib, Zohreh Faezizadeh, Masoud Godarzee
Keywords
Related Articles
- EP ID EP130239
- DOI -
- Views 116
- Downloads 0
Development and characterization of chitosan-polycarbophil interpolyelectrolyte complex-based 5-flurouracil formulations for buccal, vaginal and rectal application
The present investigation was designed with the intention to formulate versatile 5-flurouracil (5-FU) matrix tablet that fulfills the therapeutic needs that are lacking in current cancer treatment and aimed at minimizing...
Deep venous thrombosis and Atypical Antipsychotics: Three cases report.
BBackground Deep venous Thrombosis is a serious, possible life threatening event which is often ignored in psychiatric Settings. Purpose In this paper three cases of deep venous Thrombosis (DVT) following the use of olan...
Inhibition of HIV and HSV infection by vaginal lactobacilli in vitro and in vivo
Background and the purpose of the study The cervico-vaginal mucosa which is populated with microflora (mostly includes lactobacilli) is the portal of entry for sexually transmitted pathogens. methods The in vitro anti-...
Pleurodesis by erythromycin, tetracycline, AerosilTM 200, and erythromycin plus AerosilTM 200 in a rat model: a preliminary study
Background None of the current pleurodesing agents fulfil all the criteria for best pleural sclerosant. Therefore, the search for the ideal agent for chemical pleurodesis still continues. The aim of the present study wa...
Reflection on design and testing of pancreatic alpha-amylase inhibitors: an in silico comparison between rat and rabbit enzyme models
Background Inhibitors of pancreatic alpha-amylase are potential drugs to treat diabetes and obesity. In order to find compounds that would be effective amylase inhibitors, in vitro and in vivo models are usually used. T...