In vitro and In vivo Effects of the Leaf Extracts of Cassia tora and Cassia sophera in Reducing the Cytotoxicity and Angiogenesis
Journal Title: Biotechnology Journal International - Year 2013, Vol 3, Issue 3
Abstract
Aim: Consumption of C. tora and C. sophera of family caesalpinaceae as leafy vegetables by local folklore has initiated this study to investigate the cytotoxic effect and antiangiogenic effect of their leaf extracts on human cancer cell lines and chick embryo respectively. Study Design: For in vitro studies colon and hepatocarcinoma cell lines were used. For in vivo studies chicken egg chorioallantoic membrane (CAM) assay is used. Methodology: Methanol, ethyl acetate and petroleum ether extracts of leaves were tested for antiproliferative activity using MTT assay, on HCT15 and Hep G2 cell lines. In vivo CAM model was used to study anti-angiogenesis activity of extracts using filter paper discs. Results: All extracts of both plants were toxic to tested cell lines with increasing concentrations and time. Ethyl extract of Cassia tora was found to be most toxic on Hep G2 cell line, though ethyl extract and methanol extracts of both plants were found to give strong antiproliferative activity. Hep G2 was found to be most sensitive to all extracts than HCT15 as inhibited by low IC50 values. Ethyl acetate extracts of both plants exhibited greater anti-angiogenesis activity than other extracts.
Authors and Affiliations
C. Suresh, Sumangala Rao
Keywords
Related Articles
- EP ID EP237117
- DOI 10.9734/BBJ/2013/4244
- Views 119
- Downloads 0
Respiratory Burst Enzymes and Oxidant-antioxidant Status in Nigerian Children with Sickle Cell Disease
Aim: To measure respiratory burst enzymes, pro-oxidants, antioxidants and red cell indices in Nigerian children with sickle cell disease (HbSS) below five years of age and compared with apparently healthy children with n...
Characterization and Virulence of an Indigenous Soil Bacillus sp. Prospecting for Mosquito Control
Aims: Aquatic habitat of the rice fields is the effective breeding site of the mosquitoes whose overcrowding would promote their pathogens. But no attention has been paid to these habitats for isolation and identificatio...
Plant Regeneration via Somatic Embryogenesis in an Ethnomedicinal Plant Senna alata (L.) Roxb.
Aim: To study the effect of various plant growth regulators (PGRs) for induction of somatic embryogenesis and plantlet regeneration from cotyledon and leaflet explants in Senna alata (L.) Roxb. (Syn. Cassia alata) an imp...
Immediate Versus Delayed Dental Implants
Objective: The main aim of this study was to compare immediate and delayed implants for the placement and postoperative assessment. For this purpose, dental implant were placed using the set criteria and patients were re...
Evaluation of the Effects of Drying Methods on Phytochemicals in Thevetia neriifolia Parts as a Potential Rodenticide
Synthetic rodenticides are effective and rapid in controlling rats; however, they are toxic to non-target species including humans, as well as the environment. Thevetia neriifolia (Pers.) K. Schum different parts however...