In vitro Anti-Herpes simplex Type-1 Virus Evaluation of Extracts from Kenya Grown Pyrethrum (Chrysanthemum cinerariaefolium)
Journal Title: European Journal of Medicinal Plants - Year 2016, Vol 17, Issue 2
Abstract
Objective: To evaluate in vitro anti-Herpes simplex type 1 activity of methanol and aqueous crude extracts of pyrethrum (Chrysanthemum cinerariaefolium) plant grown in Kenya. Methods: Cytotoxic effect of methanol and aqueous extracts was determined on vero cells (African green monkey kidney cells) by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenylTetrazolium Bromide) reduction colorimetric assay. Antiviral effect of pyrethrum extracts was evaluated; before (post-treatment infection) virus infection to the cells and after (pre-treatment infection) virus infection to cells. Results: Methanol extract exhibited higher cytotoxicity of CC50 = 42.23 ± 0.320 µg/ml compared to aqueous extract of CC50 = 249 ± 8.4 µg/ml. Methanol extract exhibited a higher anti HSV-1 potency of IC50 = 1.69 µg/ml (TI = 24.99) than the aqueous extract of IC50 = 38.13 µg/ml (TI = 6.53) in the post-treatment infection evaluations. This might be due to its effect on cellular receptors preventing virus entry, while the aqueous extract exhibited higher virus inhibitory potency in the pre-treatment infection, of IC50 = 23.21 µg/ml (TI=10.73) compared to the methanol extract of IC50 = 11.18 µg/ml (TI =3.78), this effect could be due to its effect on some stage during HSV virus replication process. Conclusions: Crude methanol and aqueous extracts from Kenyan grown pyrethrum exhibited inhibition potency against HSV-1 in vitro on Vero cells.
Authors and Affiliations
Simon Alem, Festus M. Tolo, Nicholas Adipo, Peter G. Mwitari, Ngetich K. Japheth, Anselimo O. Makokha
Keywords
Related Articles
- EP ID EP349649
- DOI 10.9734/EJMP/2016/28967
- Views 107
- Downloads 0
Evaluation of Antistress Potential of Negundin A from Vitex negundo in Acute Stress Induced Mice
Study Background: The current study has been designed to evaluate the antistress activity of bioactive compound, Negundin A from hydroalcoholic extract of the leaves of Vitex negundo. An attempt has been made to isolate...
In vitro Cytotoxic Effects and Antibacterial Activity of Moroccan Medicinal Plants Aristolochia longa and Lavandula multifida
Aims: The aim of this study was the evaluation of in vitro cytotoxic and antibacterial activities of organic extracts from Aristolochia longa and Lavandula multifida. Study Design: Evaluation of in vitro cytotoxic and an...
Assessment of Antitheilerial Activity of the Aqueous Extract of Kigelia africana Unripe Fruits against Theileria lestoquardi
Aims: To investigate the in vitro schizonticidal activity of aqueous extract of Kigelia africana unripe fruits (Um shotour) Lam. Benth. In Hook. against Theileria lestoquardi. Study Design: In vitro screening of the aque...
Effect of Natural Cocoa Powder Supplementation on Oxidative Stress in Healthy Ghanaians
Introduction: Natural cocoa powder (NCP) has been recognized to possess significant amounts of flavonoids and methylxanthines with healthful benefits. Little or no work has been done on adult Ghanaians involving suppleme...
Ethnomedicinal Information and Phytochemical Screening of Medicinal Plants Used in the Treatment of Diarrhea in Lagos State, Nigeria
Aim: To identify and screen the phytochemicals of medicinal plants with anti-diarrhea potential in Lagos State, Nigeria. Place and Duration of the Study: Ethnobotanical survey and phytochemical screenings of plants us...