In Vitro Antibacterial Activity of Adenia Cissampeloides Plant Extracts on Bacteria Isolated from Fishes Recovered from Uwana River, Ebonyi State, Nigeria
Journal Title: The Journal of Middle East and North Africa Sciences - Year 2018, Vol 4, Issue 1
Abstract
Adenia Cissampeloides, a Pesticidal plant has dynamic active ingredients which cause physiological destruction in fishes including Clarias gariepinus. Majority of these Pesticidal plants contain medicinal values. In this study, the in vitro antibacterial activity of A. Cissampeloides plant extracts on bacteria isolated from fishes recovered from Uwana River, Ebonyi State, Nigeria was bacteriologically investigated. The isolation and identification of bacteria from the fishes recovered from Uwana River was carried out using standard microbiology techniques. The phytochemical properties of the plant extracts were studied using phytochemical analysis while the antimicrobial activities of the A. Cissampeloides plant extracts were evaluated using agar well diffusion technique. Results from physicochemical analysis of the water samples and bacterial count established that Uwana River is polluted. Fourteen species of aerobic heterotrophic Gram-positive and Gramnegative bacteria were isolated and identified from the fishes. The Gram-positive bacteria included, Staphylococcus lugdunensis, S. hominis, S. cohnii, Streptococcus pyogenes, Streptococcus pneumoniae, Kocuria varians and Gram-negative bacteria were Raoutella ornithinolytica, Klebsiella pneumoniae, Aeromonas hydrophila, Aeromonas veronii, Proteus vulgaris, Serratia fonticola, and Enterobacter Gergovia. The most frequently isolated organism from the fishes was Raoutella ornithinolytica (19.04% and 31.82%). Phytochemical analysis of the plant extracts shows that it possesses antimicrobial properties. Extract with petroleum ether showed highest inhibition zone diameter (8.2 mm) with Aeromonas veronii, while the least diameter was observed in Staphylococcus cohnii (4 mm). Methanol extracts of the plant on R. ornithinolytica showed (7mm) while on Aeromonas hydrophila it was (5mm). The results of this study show that the plant extracts of A. Cissampeloides had appreciable antimicrobial activity on bacteria isolated from fishes recovered from Uwana River. Conclusively, the tested extracts of A. Cissampeloides plant showed varying spectra of inhibitions and/or antimicrobial activity against the isolated bacteria.
Authors and Affiliations
Henrietta O. Uzoeto, Thomas E. Ayogu, Emmanuel A. Nwakaeze, Ifeanyichukwu R. Iroha, Chika Ejikeugwu
Keywords
Related Articles
- EP ID EP248725
- DOI -
- Views 104
- Downloads 0
Diagnosis of Artisan Textile Microenterprises of Tlaxcala, Mexico
This article presents a diagnosis of the current situation of artisanal textile microstate of Tlaxcala, Mex is presented. Because it is important to know about the situation they are going through, like how to market the...
Leukemic Retinopathy Mimicking Cytomegalic Virus Retinitis Ending in Total Blindness from Tumor Lysis Following Local Corticosteroid and Salvage Chemotherapy
Authors present a 15-year-old adolescent who recently received chemotherapy for T-cell acute lymphoblastic leukemia. The bilateral visual decrease resulted from diffuse superficial perivascular hemorrhages. The clinical...
The Hardy-Littlewood Prime k-tuple Conjecture is False
Using Jiang function, we prove Jiang prime k-tuple theorem, and prove that the Hardy-Littlewood prime k-tuple conjecture is false. Jiang prime k-tuple theorem can replace the Hardy-Littlewood prime k-tuple conjecture.
Integrated Energy Efficient Clustering Strategy for Wireless Sensor Networks
The foremost compensations of clustering are the communication of combined information to the base station, bids scalability for enormous quantity of nodes and lowers down energy depletion. Essentially, clustering could...
Morphometric Changes Due To β Carotene on Wistar Rats Fed Dietary Fat
Beta-carotene (βC), an antioxidant found in fruits and vegetables is responsible for the yellow color of these plants. The aim of the present research was to investigate the morphometric changes on Wistar rats fed high d...