In vitro antibacterial potential of Hydrocotyle javanica Thunb.
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2016, Vol 6, Issue 1
Abstract
Objective: To explore the in vitro antimicrobial potential of Hydrocotyle javanica (H. javanica) Thunb. against human pathogenic bacteria and to standardize purification protocol to harvest the active principle in large scale. Methods: In the present study, methanol and aqueous extracts of H. javanica were examined for in vitro antimicrobial potency against food poisoning, human pathogenic gastrointestinal and topical bacteria. Results: The methanol and aqueous extracts yielded bioactive crude extracts that have antibacterial potentiality against the human pathogenic bacteria tested. The chemical constituents of the crude extracts showed the presence of alkaloids, flavonoids, phenols, tannins, leucoanthocyanidins and cardiac glycosides in the methanol extract, and alkaloids, saponins and triterpenoids in the water extract. Further solvent fractionation and purification of the crude extracts showed that the active antibacterial compounds were alkaloid in dichloromethane fraction and phenols in methanol fraction with a very strong bactericidal activity as evidenced by SEM and lactate dehydrogenase study. Conclusions: Based on the findings it could be inferred that H. javanica would be a potential source for developing biotherapeutics against the human pathogenic bacteria. Further research work is going on in our laboratory to elucidate the structure of the active molecules.
In vitro screening of snake venom against multidrug-resistant tuberculosis
The re-emergence of multidrug-resistant tuberculosis (MDR-TB) has brought to light the importance of screening effective novel drugs. In the present study, in vitro activities of different snake (Naja naja, Bungarus fasc...
Anthelmintic activity of ethanolic leaf extract of Jasminum mesnyi
Objective: To evaluate the anthelmintic activity of ethanolic extract of leaves of Jasminum mesnyi. Methods: Anthelmintic activity was carried out on adult Indian earthworm Eisenia fetida. In this study, 20 mg/mL and 40...
Novel insights into identifiation of shedders and transmitters of avian leukosis virus
Avian leukosis virus (ALV) infection is one of the most common infections in domestic poultry. Even though loss due to mortality is only 1%-2%, it causes drastic decline in production because of subclinical infections. E...
Phlebotomine fauna in the Ponta Porã city: epidemiological importance in border line between Brazil and Paraguay
Objective: To identify the urban phlebotomine sandfly fauna in Ponta Por觔, Mato Grosso do Sul State, Brazil. Methods: The captures were undertaken from April 2009 to March 2010 with CDC light traps in 14 ecotopes (intra...
Hepatoprotective activity of Spillanthes acmella Extracts against CCl4- induced liver toxicity in rats
Objective: Hepato protective activity of Spillanthes acmella Extracts (70% ethanol, methanol, ethyl acetate and hexane) were investigated. Methods: Hepatoprotective activity was assessed against CCl4 induced liver intoxi...