In vitro Antimicrobial Activities of Crude Extracts of Vernonia amygdalina and Croton macrostachyus against Some Bacterial and Fungal Test Pathogens
Journal Title: The Journal of Phytopharmacology - Year 2019, Vol 8, Issue 2
Abstract
The aim of the present study was to investigate the antibacterial and antifungal activities of crude extracts of Vernonia amygdalina and Croton macrostachyus against four bacterial pathogenic species (i.e. Staphylococcus aureus, Streptococcus agalactiae, Salmonella typhi, Escherichia coli) and two fungal pathogenic species (i.e. Aspergillus flavus and Aspergillus niger). Antimicrobial activities of the resulting extracts were determined by measuring zone of inhibitions on cultures of six selected pathogens using the disc diffusion method. The minimum inhibitory concentration (MIC) of the two plant extracts against the same pathogens was determined using the broth dilution method. Chloroamphenicol was used as positive control for for bacterial pathogen and Tilt was also used as positive controls fungal pathogens. Meanwhile Dimethyl Sulfoxide was taken as a negative control for both. Highest percentage yield was recorded for methanol extract of V. amygdalina (14.89±0.25%) and the lowest yield was observed for the water extract of V. amygdalina (4.58±0.27). All the methanolic and ethanolic crude extracts had the highest growth inhibitory effects as compared with the water crude extracts on all bacterial pathogens. The methanol crude extract of C. macrostachyus had highest zone of inhibition (21±0.29 mm) against S. aureus, with a minimum inhibitory concentration of 25mg/ml. Similarly, the ethanol crude extract of C. macrostachyus had shown highest antifungal activity against A. niger with inhibition zone of 15.5±0.29. The results indicate the potential of these medicinal plants in treating some bacterial and fungal infection. Thus, further detailed studies are recommended to be carried out to characterize their bioactive compounds for development of new effective antimicrobial drugs.
Authors and Affiliations
Selam Habtom, Sibhatu Gebrehiwot
Keywords
Related Articles
- EP ID EP49865
- DOI https://search.crossref.org/?q=10.31254%2Fphyto.2019.8206
- Views 216
- Downloads 0
Influence of microwave assisted extraction on antioxidant and antiplasmodial activities of Trichilia roka extracts
Antiplasmodial and antioxidant activities of extracts derived from Trichilia roka (Chiov) (Meliaceae) root bark were determined respectively in vitro and using two methods as well as 1,1-diphenyl-2-picrylhydrazyl radica...
Natural dyeing of fabrics using Quercus robur L. (fruit cups) dye and Punica granatum L. (peel) mordant
Investigation was carried out to study the dyeing quality of Quercus robur L. (fruit cups) and Punica granatum L. (peel) mordant for the dyeing of wool and cotton fabrics. The fabrics were dyed both excluding and inclu...
A comparative study of phytochemical profile and in vitro antioxidant activities of dark and light dried fig (Ficus carica L.) varieties
To study bioactive compounds of the dried fig, nine varieties (3 dark and 6 light varieties) were analyzed for their phytochemical profile and antioxidant activities. The antioxidant activity was evaluated using four m...
Anxiolytic activity of ethanolic extract of aerial parts of Tribulus terrestris in mice
Herbs have always been a preferred choice of treatment for people of the subcontinent and the Indo-Pak subcontinent has a long tradition of the use of herbs as medicines. In the given study ethanolic extract of Tribulu...
Evaluation of antioxidant activity of some wild edible fruits collected from Boda and Kolli hills
Free radicals or highly reactive oxygen species are capable of inducing oxidative damage to human body. Antioxidants are the compounds which terminate the attack of reactive species and reduce the risk of diseases; hen...