IN VITRO ANTIOXIDANT ACTIVITY ASSESSMENT ON ALCOHOLIC EXTRACT OF CYANOBACTERIA CULTURES FROM SPIRULINA PLATENSIS
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2013, Vol 2, Issue 3
Abstract
The antioxidant supplementation such as vitamin C, E, A and beta carotene etc, has proven to prevent the process of ageing caused by free radicals. Antioxidants either prevent free radical production or counteract the impeding damage by doing so they are involved in the protection and prevention of cellular damage which is the common pathway for cancer, aging and much disease. The cyanobacteria is a blue green micro algae believed to be a rich antioxidant source, found in abundance in Spirulina platensis is evaluated for antioxidant potential by modern in vitro techniques. METHODS the alcoholic extract of cyanobacteria is studied by in vitro Lipid Peroxidation inhibition assay (LPO) , The nitric oxide (NO)free radical scavenging activity and the determination of the DPPH (one, 1-diphenyl-2- picrylhydrazil) radical scavenging activity in concentrations which ranged from 1.95 g/mg to 500 g/mg in a geometric progression using spectrophotometer Results: The IC-50 % inhibition value of cyanobacteria by LPO is 102.70 mcg/ml, DPPH is 63.35 mcg/ml and by NO is 8.80 mcg/ml CONCLUSION The alcoholic extract of corynebacterium culture from Spirulina platensis exhibited potent NO free radical scavenging activity with mild activity on DPPH and LPO inhibition assay.
Authors and Affiliations
Senthilkumaran Jagadeesh J| Deparetment of Pharmacology, Sree Balaji Medical College and Hospital, Tamil Nadu, India, Corresponding author email:jagadeesh.j@gmail.com, Jaiganesh K| Department of Physiology, Mahatma Gandhi Medical College and Research Institute Pillaiyarkuppam, Pondicherry, India, Somasundaram G| Department of Pharmacology, Mahatma Gandhi Medical College and Research Institute Pillaiyarkuppam, Pondicherry, India
Keywords
Related Articles
- EP ID EP11027
- DOI 10.5958/j.2319-5886.2.3.108
- Views 357
- Downloads 22
HEPATIC ADENOMA IN AN ELDERLY MALE PATIENT: A RARE CASE REPORT
Hepatocellular adenomas are usually affect females in their 3rd and 4th decades of life. Uses of oral contraceptives use or anabolic steroids have been blamed for this. This is rare in old age and in an elderly male pr...
Pharmacoeconomic Evaluation, Cost Minimization Analysis of Anti-Diabetic Therapy in Gujarat
Economic burden of DM was approximated $ 132 billion in 2002. 55 patients with DM were recruited. Their clinical information was analyzed for most commonly and most costly prescribed drug molecule. Three brands of both...
Effects of Short Term Supplementation of Fish Oil Capsules on the Blood Fatty Acid Profle of Vegetarians: A Pilot Study
Background and Aims: Omega-3 fatty acids are essential for cardio vascular as well as overall health. Therefore, fsh oil which is a rich source of omega-3 fatty acids namely EPA and DHA is recommended. We supplemented v...
IMPACT OF CLINICAL PLACEMENT ON RADIOGRAPHY STUDENTS IN GHANA
Background: The clinical setting is one of the most valuable resources available to training institutions to prepare students to competently care for patients and also execute certain tasks with little or no supervision...
ROLE OF COMPUTED TOMOGRAPHY IN EVALUATION OF PANCREATIC DISEASES
Context: The evolving role of CT in the study of pancreas is not only in its ability to directly define the presence of an abnormality but it also surpasses the other imaging modalities in being able to demonstrate the...