In-vitro Antioxidative Property of Water Soluble Bacterial Melanin and Evaluation on Novel Model System
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2014, Vol 2, Issue 12
Abstract
Melanin is important because it’s the most primitive and universal pigment in living organisms. The present study reports antioxidant activity of melanin pigment from EP83 a Gram negative bacterial strain isolated from garden soil, of Gujarat University Campus, Gujarat, India. The purified melanin fraction showed promising free radical scavenging activity through DPPH, Superoxide Radical Scavenging Activity, Lipid Peroxidation Inhibition Assay, ABTS+ scavenging capacity assay methods and also onto its evaluation of fish mince model system. The colored DPPH solution was progressively reduced to half during the course of incubation of 54, 21, 16 hours for 14.9, 29.8 and 44.7μg/mL of melanin concentrations respectively and ABTS+ scavenging capacity of the extract was 26.49 ± 2.34%, 37.24 ± 2.96% and 43.88 ± 2.92% of control for the melanin extract at the concentrations of 10, 15 and 20% (v/v), of melanin respectively. The superoxide anion radical scavenging assayed by both PMSNADH system and fish mince system reveled dose dependent scavenging activity by the n-butanol extract. Melanin extract also scavenges ABTS+ free radical significantly in a dose as well as time dependent manner.
Authors and Affiliations
E. A. Pithawala, N. K. Jain
Keywords
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