In vitro callogenesis for the induction of somatic embryogenesis and antioxidant production in Eugenia uniflora
Journal Title: Biotecnología Vegetal - Year 2020, Vol 20, Issue 2
Abstract
Eugenia uniflora is an American tree species with ecological, agronomical and medicinal importance. However, just few studies have focused on the in vitro propagation and production of secondary metabolites. This study investigated the explant sources and culture conditions for the in vitro callogenesis in E. uniflora towards induction of somatic embryogenesis and production of antioxidant compounds. Nodal segments, leaf sections and root segments from in vitro germinated seeds were used as explants and eight different combinations of auxins (2,4-D or NAA) and cytokinins (BAP or TDZ) were tested for the callus induction. The best callogenic response was observed in nodal segments, followed by leaf sections. Root segments presented comparatively poorer callogenic performance. Calli from nodal segments cultivated in MS medium with NAA (10 μM) + TDZ (5 μM) originated putative pro-embryogenic structures, while the culture in liquid medium using MS supplemented with NAA (10 μM) + TDZ (5 μM) revealed significantly higher content of phenols and flavonoids, as well as higher reducing capacity than the tested treatments and the control (fresh leaves). In summary, the calli obtained from nodal segments revealed competence for somatic embryogenesis induction and development as well as the production of secondary metabolites with pharmaceutical potential.
Authors and Affiliations
Valdir Marcos Stefenon, Marcos Vinicius Marques Pinheiro, Fábio Ribeiro de Freitas, Viliano José Boing da Silva, Patrícia de Brum Vieira, Daniele Damian dos Santos, Miguel Pedro Guerra
Keywords
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